The human bone marrow harbors a CD45− CD11B+ cell progenitor permitting rapid microglia-like cell derivative approaches
| Autor: | Tomas Deierborg, Amelie Tison, Johan Bengzon, Tania Ramos-Moreno, Anna Giorgia Hjelmér, Antonio Boza-Serrano, Andreas Bruzelius, Isabel Hidalgo |
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| Přispěvatelé: | Royal Physiographic Society of Lund, Crafoord Foundation, Per-Eric and Ulla Schyberg's Foundation, Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Central Nervous System Stromal cell Pluripotent stem cell microglia‐like cell in vitro model Biology 03 medical and health sciences 0302 clinical medicine Human bone marrow medicine Humans Bone marrow Yolk sac Progenitor cell lcsh:QH573-671 Induced pluripotent stem cell Progenitor lcsh:R5-920 CD11b Antigen Microglia lcsh:Cytology Microglia-like cell in vitro model Monocyte Stem Cells Microglial precursor Cell Biology General Medicine Common myeloid progenitor Cell biology Standards Protocols Policies and Regulations for Cell‐based Therapies 030104 developmental biology medicine.anatomical_structure Primitive myeloid progenitor Leukocyte Common Antigens lcsh:Medicine (General) 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells Translational Medicine, Vol 10, Iss 4, Pp 582-597 (2021) idUS. Depósito de Investigación de la Universidad de Sevilla instname Stem Cells Translational Medicine Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 2157-6580 2157-6564 |
| Popis: | Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches. This work was supported by Craafordska Stiftelsen, the Royal Physiographic Society of Lund, Per Eric and Ulla Shyberg's Foundation, and Viveca Jeppsson. |
| Databáze: | OpenAIRE |
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