Expression of the eight GABAA receptor α subunits in the developing zebrafish central nervous system
| Autor: | Hanna E. Dorman, Daniel R. Turkewitz, Abigail E. Case, Jon J. McClain, Bryan Monesson-Olson, Gerald B. Downes, Aaron B. Steiner |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Central Nervous System Subfamily lcsh:Medicine Artificial Gene Amplification and Extension Nervous System Biochemistry Polymerase Chain Reaction 0302 clinical medicine Animal Cells Medicine and Health Sciences Protein Isoforms Tissue Distribution Receptor lcsh:Science Zebrafish In Situ Hybridization Phylogeny Data Management Regulation of gene expression Neurons 0303 health sciences Multidisciplinary GABAA receptor Brain Gene Expression Regulation Developmental Eukaryota Neurochemistry Phylogenetic Analysis Animal Models Neurotransmitters Olfactory Bulb Cell biology Phylogenetics Spinal Cord Experimental Organism Systems Osteichthyes Multigene Family Vertebrates Anatomy Cellular Types medicine.drug Research Article Gene isoform Computer and Information Sciences animal structures Protein subunit In situ hybridization Biology Research and Analysis Methods gamma-Aminobutyric acid Retina Olfactory Receptor Neurons 03 medical and health sciences Model Organisms medicine Animals Evolutionary Systematics Molecular Biology Techniques Gene Molecular Biology 030304 developmental biology Taxonomy Evolutionary Biology lcsh:R Organisms Biology and Life Sciences Afferent Neurons Cell Biology Reverse Transcriptase-Polymerase Chain Reaction Zebrafish Proteins Gamma-Aminobutyric Acid biology.organism_classification Receptors GABA-A Hindbrain Neuroanatomy 030104 developmental biology Fish nervous system Cellular Neuroscience lcsh:Q 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 4, p e0196083 (2018) |
| ISSN: | 1932-6203 |
| Popis: | GABA is a robust regulator of both developing and mature neural networks. It exerts many of its effects through GABAA receptors, which are heteropentamers assembled from a large array of subunits encoded by distinct genes. In mammals, there are 19 different GABAA subunit types, which are divided into the α, β, γ, δ, ε, π, θ and ρ subfamilies. The immense diversity of GABAA receptors is not fully understood. However, it is known that specific isoforms, with their distinct biophysical properties and expression profiles, tune responses to GABA. Although larval zebrafish are well-established as a model system for neural circuit analysis, little is known about GABAA receptors diversity and expression in this system. Here, using database analysis, we show that the zebrafish genome contains at least 23 subunits. All but the mammalian θ and ε subunits have at least one zebrafish ortholog, while five mammalian GABAA subunits have two zebrafish orthologs. Zebrafish contain one subunit, β4, which does not have a clear mammalian ortholog. Similar to mammalian GABAA receptors, the zebrafish α subfamily is the largest and most diverse of the subfamilies. In zebrafish there are eight α subunits, and RNA in situ hybridization across early zebrafish development revealed that they demonstrate distinct patterns of expression in the brain, spinal cord, and retina. Some subunits were very broadly distributed, whereas others were restricted to small populations of cells. Subunit-specific expression patterns were similar between zebrafish, frogs and rodents, which suggests that the roles of different GABAA receptor isoforms are largely conserved among vertebrates. This study provides a platform to examine isoform specific roles of GABAA receptors within zebrafish neural circuits and it highlights the potential of this system to better understand the remarkable heterogeneity of GABAA receptors. |
| Databáze: | OpenAIRE |
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