Autoradiographic identification of a gastrin receptor on the human parietal cell
| Autor: | Mel Praissman, Laura A. Praissman, Aram Manonkian, Bernard P. Lane, Yu-Min Lu, Mary Ellen Fay, Douglas L. Brand, Mae Walden |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male medicine.medical_specialty Physiology Clinical Biochemistry Regulation of gastric function Biology digestive system Biochemistry Cellular and Molecular Neuroscience Endocrinology Parietal Cells Gastric Internal medicine Gastrins medicine Radioligand Humans Enterochromaffin-like cell Parietal cell Gastrin Binding Sites digestive oral and skin physiology Middle Aged Molecular biology Gastric chief cell medicine.anatomical_structure Autoradiography Gastric acid Female Receptors Cholecystokinin G cell Digestive System hormones hormone substitutes and hormone antagonists |
| Zdroj: | Regulatory Peptides. 73:183-190 |
| ISSN: | 0167-0115 |
| DOI: | 10.1016/s0167-0115(98)00003-2 |
| Popis: | Gastrin plays an important role in regulating gastric acid secretion and gastrointestinal mucosal growth but its cellular sites of action in man have not been determined. Using cryostat sections of gastric mucosal tissue we have identified (125I-gastrin binding followed by fixation-wet emulsion autoradiography) and characterized (125I-gastrin binding followed by counting) a gastrin receptor binding site in the human stomach. This site displayed binding characteristics similar to those observed in isolated cell systems: specifically, 125I-gastrin binding was rapid (t1/2 approximately 10 min at 37 degrees C), temperature-dependent (3.5 fold more radioligand bound at 22 degrees C than at 4 degrees C) and saturable. The binding of the radioligand was also tissue specific and was five-fold greater in the gastric body than in the gastric antrum and duodenum. In the autoradiographs, silver grains were localized only to parietal cells and not to other epithelial cell types. In the presence of 40 nM gastrin grains were no longer present over parietal cells demonstrating that these sites were both saturable and of high affinity. These data provide the first demonstration of gastrin binding sites (putative receptors) on parietal cells in the human stomach and suggest that gastrin acts directly on these cells to help regulate gastric acid secretion and/or mucosal growth. |
| Databáze: | OpenAIRE |
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