Effects of lecithin: Cholesterol acyltransferase genotypes, enzyme levels, and activity on high-density lipoprotein levels
| Autor: | Cenk Aral, Mehmet Agirbasli, Mutlu Sumerkan, Sukru Aksoy, Deniz Agirbasli, Beyazit Cirakoglu, Fatih Eren |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Blood Glucose Male medicine.medical_specialty food.ingredient Genotype Turkey Endocrinology Diabetes and Metabolism Sterol O-acyltransferase Polymorphism Single Nucleotide Lecithin Body Mass Index Phosphatidylcholine-Sterol O-Acyltransferase chemistry.chemical_compound food High-density lipoprotein Gene Frequency Internal medicine Internal Medicine Humans Medicine Triglycerides Aged chemistry.chemical_classification Nutrition and Dietetics biology business.industry Cholesterol HDL Cholesterol LDL Metabolism Middle Aged Enzyme assay Enzyme Endocrinology chemistry Cardiovascular Diseases biology.protein Population study Female lipids (amino acids peptides and proteins) Cardiology and Cardiovascular Medicine business Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Lipidology. 5:152-158 |
| ISSN: | 1933-2874 |
| Popis: | Background Lecithin:cholesterol acyltransferase (LCAT) is one of the key enzymes controlling cholesterol homeostasis and plays a primary role in high-density lipoprotein cholesterol (HDL-C) maturation. Objective The aim of our study was to evaluate the effects of LCAT gene polymorphisms 511C/T (exon4), 4886C/T (rs5923), and 608C/T (rs5922) on LCAT enzyme level, activity, and HDL-C levels. Methods The study population was selected from consecutive subjects with low ( 65 mg/dL) seen in our lipid clinic. LCAT polymorphisms were analyzed with a restriction fragment length polymorphism assay. LCAT activity and levels were measured by colorimetric enzymatic and enzyme-linked immunoassay methods, respectively. Results The 4886C/T polymorphism was the most commonly observed variant of LCAT gene. T-allele frequencies in subjects with low (n = 50) and high (n = 50) HDL-C were 0.54 and 0.37, respectively ( P = .019). TT genotype was more common among low HDL-C group (30% vs 14%, P = .05). The effects of LCAT enzyme appeared to depend on the HDL-C level. In subjects with low HDL-C, LCAT enzyme levels correlated positively with body mass index ( P r = 0.544), HDL-C ( P = .006, r = 0.404), triglycerides ( P = .001, r = 0.487), total cholesterol ( P r = 0.541), and low-density lipoprotein-cholesterol ( P = .001, r = 0.477) levels. LCAT activity correlated positively with fasting glucose levels ( P = .008, r = 0.390). Conclusion LCAT genotype, enzyme level, and activity modulate HDL-C metabolism, particularly among subjects with low HDL-C levels. |
| Databáze: | OpenAIRE |
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