Colorimetric Assay for Guaiacol O-Methyltransferase
| Autor: | John O. Macfarlane, John W. Lee, C. C. Cheng |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Catechol
biology Chemistry Guaiacol Pharmaceutical Science Substrate (chemistry) Methyltransferases Phenolic acid Hydrogen-Ion Concentration O-methyltransferase Colorimetry (chemical method) Structure-Activity Relationship chemistry.chemical_compound Methods Side chain biology.protein Organic chemistry Colorimetry Sulfanilic acid |
| Zdroj: | Journal of Pharmaceutical Sciences. 65:1689-1690 |
| ISSN: | 0022-3549 |
| Popis: | A relatively simple colorimetric assay was developed for guaiacol O-methyltransferase. Monomethylated phenolic acid substrates are enzymatically methylated to their corresponding dimethoxyl compounds; S-adenosylmethionine serves as the methyl donor. Enzymatic activity is measured by colorimetric assay of the monomethylated (nonreacted) substrate by coupling of the phenolic acid with a diazotized sulfanilic acid. The methoxyl and hydroxyl groups may be in the 3,4- or 4,3-positions on the substrate molecules; however, a side chain (COOH) or a substituted side chain is necessary for enzyme action. The radioactive demonstration of guaiacol O-methyltransferase as a separate entity from catechol O-methyl-transferase was confirmed. |
| Databáze: | OpenAIRE |
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