Phospholipid composition of in vitro endothelial microparticles and their in vivo thrombogenic properties
| Autor: | D.G. Meuleman, G.M.T. Vogel, Rienk Nieuwland, A. Sturk, Anita N. Böing, F.J. Hoek, M. N. Abid Hussein, E. Biro |
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| Přispěvatelé: | Other departments, Laboratory for Experimental Clinical Chemistry, Laboratory for General Clinical Chemistry, Amsterdam Cardiovascular Sciences, Cancer Center Amsterdam |
| Rok vydání: | 2008 |
| Předmět: |
Time Factors
Phospholipid Biology Umbilical vein Thromboplastin chemistry.chemical_compound Tissue factor In vivo Interleukin-1alpha Animals Humans Particle Size Blood Coagulation Cells Cultured Chromatography High Pressure Liquid Phospholipids Phosphatidylethanolamine Thrombin Endothelial Cells Thrombosis Hematology Phosphatidylserine Molecular biology In vitro Rats Endothelial stem cell Biochemistry chemistry Models Animal |
| Zdroj: | Thrombosis research, 121(6), 865-871. Elsevier Limited |
| ISSN: | 0049-3848 |
| Popis: | Introduction: Microparticles from activated endothelial cells (EMP) are well known to expose tissue factor (TF) and initiate coagulation in vitro. TF coagulant activity is critically dependent on the presence of aminophospholipids, such as phosphatidylserine (PS) and phosphatidylethanolamine (PE), but it is unknown whether or not TF-exposing EMP are enriched in such aminophospholipids. Furthermore, despite the fact that EMP have been reported in several pathological conditions, direct evidence for their (putative) coagulant properties in vivo is stilt lacking. We investigated the phospholipid composition of endothelial MP (EMP) and their thrombogenic properties in vivo. Materials and methods: Human umbilical vein endothelial cells (HUVEC; n=3) were incubated with or without interleukin (IL)-1 alpha (5 ng/mL; 0-72 h). Phospholipid composition of EMP was determined by high-performance thin layer chromatography. The association between EMP, TF antigen and activity was confirmed in vitro (ELISA, Western blot and thrombin generation). Thrombogenic activity of EMP in vivo was determined in a rat venous stasis model. Results: Levels of TF antigen increased 3-fold in culture medium of IL-1 alpha-treated cells (P |
| Databáze: | OpenAIRE |
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