Phenylobacterium terrae sp. nov., isolated from a soil sample of Khyber-Pakhtun-Khwa, Pakistan
| Autor: | Xing Huang, Nasr Ullah Khan, Xiao-Yang Zhi, Inam Ullah Khan, Iftikhar Ahmed, Min Xiao, Wan-Taek Im, Wen-Jun Li, Neeli Habib |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Phenylobacterium composti 030106 microbiology Phenylobacterium Microbiology 03 medical and health sciences food RNA Ribosomal 16S Pakistan Molecular Biology Phylogeny Soil Microbiology Alphaproteobacteria Phenylobacterium immobile Strain (chemistry) biology Phylogenetic tree General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Molecular Typing genomic DNA Phenotype 030104 developmental biology Bacteria |
| Zdroj: | Antonie van Leeuwenhoek. 111:1767-1775 |
| ISSN: | 1572-9699 0003-6072 |
| DOI: | 10.1007/s10482-018-1064-2 |
| Popis: | A Gram-stain negative, aerobic, motile, non-spore-forming and rod-shaped bacterial strain, designated YIM 730227T, was isolated from a soil sample, collected from Karak district, Khyber-Pakhtun-Khwa, Pakistan. The bacterium was characterized using a polyphasic taxonomic approach. Pairwise comparison of the 16S rRNA gene sequences showed that strain YIM 730227T is closely related to Phenylobacterium lituiforme FaiI3T (97.5% sequence similarity), Phenylobacterium muchangponense A8T (97.4%), Phenylobacterium panacis DCY109T (97.1%), Phenylobacterium immobile ET (97.1%) and Phenylobacterium composti 4T-6T (97.0%), while also sharing 98.0% sequence similarity with Phenylobacterium hankyongense HKS-05T after NCBI blast, showing it represents a member of the family Caulobacteraceae. The major respiratory quinone was Q-10 and the major fatty acids were C16:0, summed feature 8 (comprising C18:1ω7c and/or C18:1ω6c), C18:1ω7c 11-methyl and C17:0. The polar lipids were phosphatidylglycerol, unidentified glycolipids, phospholipid and unidentified lipid. The G + C content of the genomic DNA was 68.2 mol%. The DNA–DNA relatedness values of strain YIM 730227T with P. hankyongense HKS-05T, P. lituiforme FaiI3T, P. muchangponense A8T, P. panacis DCY109T, P. immobile ET and P. composti 4T-6T were 31.3 ± 0.6, 26.1 ± 0.2, 24.3 ± 0.1, 21.8 ± 0.9, 19.8 ± 0.6 and 18.2 ± 1.1%, respectively, values lower than 70%. Besides the morphological and chemotaxonomic characteristics, phylogenetic analyses of 16S rRNA gene sequences and the biochemical characteristics indicated that the strain YIM 730227T represents a novel member of the genus Phenylobacterium, for which the name Phenylobacterium terrae sp. nov. (type strain YIM 730227T = KCTC62324T = CGMCC 1.16326T) is proposed. |
| Databáze: | OpenAIRE |
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