Screening for gestagens in kidney fat using accelerated solvent extraction and liquid chromatography electrospray tandem mass spectrometry
| Autor: | H. Hooijerink, Mirjam Nielen, E.O van Bennekom |
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| Rok vydání: | 2003 |
| Předmět: |
Detection limit
Electrospray Chromatography Chemistry medicine.medical_treatment Extraction (chemistry) Supercritical fluid extraction Tandem mass spectrometry Biochemistry supercritical-fluid extraction Analytical Chemistry Steroid medicine Environmental Chemistry tissues Sample preparation BU Microbiologische & Chemische Voedselanalyse Solid phase extraction Spectroscopy BU Microbiological & Chemical Food Analysis steroids |
| Zdroj: | Analytica Chimica Acta 483 (2003) 1-2 Analytica Chimica Acta, 483(1-2), 51-59 |
| ISSN: | 0003-2670 |
| Popis: | A screening method has been developed for the determination of various anabolic steroids in kidney fat. Fat samples are extracted and steroids are trapped “on-line” during accelerated solvent extraction (ASE). Following this initial extraction samples are further purified with C18 solid-phase extraction (SPE). Compounds were finally analyzed by liquid chromatography (LC) coupled with tandem mass spectrometry (MS). The method was validated using blank kidney fat and fat samples fortified at 2 ng g−1. The detection capability (CCβ) was |
| Databáze: | OpenAIRE |
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