Effects of TNF-α and IL-10-819 TC single nucleotide polymorphisms on urogenital schistosomiasis in preschool children in Zimbabwe
| Autor: | Arthur Vengesai, Amos Marume, Theresa Chimponda, Caroline Mushayi, Takafira Mduluza, Jaclyn K. Mann |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
030231 tropical medicine
Clinical Biochemistry Schistosomiasis Single-nucleotide polymorphism susceptibility 03 medical and health sciences protective immunity 0302 clinical medicine Genotype medicine 030212 general & internal medicine Genotyping Original Research Schistosoma haematobium biology business.industry Public Health Environmental and Occupational Health Interleukin medicine.disease biology.organism_classification cytokines Genotype frequency Medical Laboratory Technology Interleukin 10 Immunology Public aspects of medicine RA1-1270 business polymorphisms |
| Zdroj: | African Journal of Laboratory Medicine African Journal of Laboratory Medicine, Vol 10, Iss 1, Pp e1-e7 (2021) |
| ISSN: | 2225-2002 |
| Popis: | Background: Knowledge gaps exist between host genetic factors and susceptibility to schistosomiasis. Objective: This study determined cytokine levels and single nucleotide polymorphisms of tumour necrosis factor (TNF)-α (rs1800629) and interleukin (IL)-10 (rs1800871) and their possible impact on susceptibility to schistosomiasis in preschool-age children in the Madziva area of Shamva district, Mashonaland Central province, Zimbabwe. Methods: Urogenital schistosomiasis was diagnosed using the urine filtration method, while a sandwich enzyme-linked immunosorbent assay was used for cytokine level determination. The survey was done in August 2015 and reinfection levels post treatment were assessed at 3, 6 and 12 months. Amplification refractory mutation system polymerase chain reaction with visualisation on 2% agarose gel electrophoresis was used for genotyping. Results: Schistosomiasis prevalence was found to be 10.5% (59/563). Reinfections were detected in only six children at 3 months and only one was reinfected at 12 months. There were no significant differences in TNF-α-308 G/A allele or genotype frequencies between the Schistosoma haematobium infected participants (p = 0.360) and uninfected participants (p = 0.279). However, no children with the IL-10-819 TT genotype had schistosomiasis. The TNF-α GG genotype corresponded with significantly lower TNF-α levels when compared with the GA or AA genotypes (p 0.001), and TNF-α levels were significantly lower in infected children compared to uninfected children (p 0.001). Conclusion: Higher TNF-α levels and lower IL-10 levels are potentially protective against schistosomiasis infection. The IL-10-819 TT genotype is potentially protective against infection through its association with lower IL-10 levels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|