Heavy and light chain sequences of four monoclonal antibodies that bind galactosylgloboside (GalGb4)
| Autor: | Li-Yuan Yu-Lee, Donald M. Marcus, Nan-Ping Weng, James G. Snyder |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Stage-Specific Embryonic Antigens
Glycosylation medicine.drug_class Molecular Sequence Data Immunology Immunoglobulin Variable Region Immunoglobulin light chain Monoclonal antibody Polymerase Chain Reaction Glycosphingolipids Immunoglobulin kappa-Chains Mice chemistry.chemical_compound Antibody Specificity medicine Animals Immunology and Allergy Antigens Tumor-Associated Carbohydrate Nucleotide Amino Acid Sequence Gene chemistry.chemical_classification Base Sequence Genes Immunoglobulin biology Nucleic acid sequence Antibodies Monoclonal Molecular biology Amino acid Immunoglobulin M chemistry Biochemistry biology.protein Binding Sites Antibody Antibody Glycoconjugates |
| Zdroj: | European Journal of Immunology. 20:2673-2677 |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | We recently described IgM monoclonal antibodies directed against the glycospingolipid galactosylgloboside (GalGb4; Marcus, M. D. et al., Arch. Biochem. Biophys, 1988.262: 620). We now present the nucleotide and deduced amino acid sequences of the heavy and light chains of these antibodies. The antibodies were generated in a single fusion, their heavy as well as their light chains are almost identical, and they appear to be clonally related. The light chains were encoded by J kappa 5 and a V kappa gene belonging to the Ox1 family, but they are only 93% homologous to the most closely related germ-line gene, and they are probably encoded by a germ-line gene that has not yet been identified. The heavy chains were all encoded by VH441 and JH2, and have identical N segments. The VH441 germ-line gene encodes a potential glycosylation site at Asn58 in the complementarity-determining region 2. This site, which has been retained in all VH441-encoded monoclonal antibodies sequenced previously, was mutated out by a single base change in all four anti-GalGb4 antibodies. |
| Databáze: | OpenAIRE |
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