Distribution and Abundance of Archaea in South China Sea SpongeHoloxeasp. and the Presence of Ammonia-Oxidizing Archaea in Sponge Cells

Autor:	Minqi Han, Fang Liu, Fengli Zhang, Baohua Zhang, Zhiyong Li
Rok vydání:	2011
Předmět:	Article Subject biology Ecology lcsh:Other systems of medicine Ammonia monooxygenase lcsh:RZ201-999 16S ribosomal RNA biology.organism_classification Sponge Complementary and alternative medicine Abundance (ecology) Crenarchaeota Botany Nitrification Nitrogen cycle Research Article Archaea
Zdroj:	Evidence-Based Complementary and Alternative Medicine, Vol 2011 (2011) Evidence-based Complementary and Alternative Medicine : eCAM
ISSN:	1741-4288 1741-427X
Popis:	Compared with bacterial symbionts, little is known about archaea in sponges especially about their spatial distribution and abundance. Understanding the distribution and abundance of ammonia-oxidizing archaea will help greatly in elucidating the potential function of symbionts in nitrogen cycling in sponges. In this study, gene libraries of 16S rRNA gene and ammonia monooxygenase subunit A (amoA) genes and quantitative real-time PCR were used to study the spatial distribution and abundance of archaea in the South China Sea spongeHoloxeasp. As a result,Holoxeasp. specific AOA, mainly group C1a (marine group I:Crenarchaeota) were identified. The presence of ammonia-oxidizing crenarchaea was observed for the first time within sponge cells. This study suggested a close relationship between sponge host and its archaeal symbionts as well as the archaeal potential contribution to sponge host in the ammonia-oxidizing process of nitrification.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a690d00e92454af61ba731ffe99fb28 https://doi.org/10.1155/2011/723696 Zobrazit plný text záznamu Plný text