Migration Groups: A Poorly Explored Point of View for Genetic Damage Assessment Using Comet Assay in Human Lymphocytes
| Autor: | Mario Alberto Ruiz-López, Erandi Arvizu-Hernández, Alexis Gerardo Sámano-León, Mónica Reynoso-Silva, Carlos Alvarez-Moya, Hugo Castañeda-Vásquez, Rafael Ramírez-Velasco |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Technology animal structures DNA damage QH301-705.5 QC1-999 tail length groups Negative control Chemical interaction medicine.disease_cause complex mixtures 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine comet assay medicine Bioassay General Materials Science Biology (General) bioassays Instrumentation QD1-999 Fluid Flow and Transfer Processes Chemistry Process Chemistry and Technology Physics genotoxicity General Engineering food and beverages Methane sulfonate Engineering (General). Civil engineering (General) Molecular biology Computer Science Applications Comet assay human lymphocytes 030104 developmental biology 030220 oncology & carcinogenesis sense organs biological phenomena cell phenomena and immunity TA1-2040 DNA Genotoxicity |
| Zdroj: | Applied Sciences, Vol 11, Iss 4094, p 4094 (2021) Applied Sciences Volume 11 Issue 9 |
| ISSN: | 2076-3417 |
| Popis: | A new point of view for genetic damage assessment using the comet assay is proposed based on the number of migration groups, the number of comets in each group, and the groups with the highest number of comets. Human lymphocytes were exposed to different concentrations of Methyl Methane Sulfonate (MMS), Maleic Hydrazide (MH), 2,4-Dichlorophenoxyacetic (2,4-D), and N-nitroso diethylamine (NDEA). Using comet assay, the migration means of the comets were determined and later grouped arbitrarily in migration groups with no higher differences than 1 µc. The number of migration groups, the number of comets in each group, and the groups with the highest number of comets (modes) were determined. All four of the genotoxic agents studied showed a significant increase (p < 0.05) in the tail length and the number of migration groups compared to the negative control. The number of migration groups did not show a significant variation between the four-genotoxic agents nor within their different concentrations. However, the comparison of the modes did show differences between the genotoxic agents, but not within the concentrations of a same genotoxic agent, which indicated a determined chemical interaction on the DNA. These parameters can improve the detection of genetic damage associated with certain genotoxic agents. |
| Databáze: | OpenAIRE |
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