Differential Expression of Tissue Inhibitor of Metalloproteinases-2 by Cutaneous Squamous and Basal Cell Carcinomas
| Autor: | Christine Wagner, Manfred Goos, Stephan Wagner, H. Peter Soyer, Hans M. Ockenfels |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Skin Neoplasms
Stromal cell Transcription Genetic Gene Expression Dermatology In situ hybridization Biology Matrix metalloproteinase Biochemistry Metastasis Extracellular matrix In vivo medicine Humans Protease Inhibitors RNA Messenger Northern blot Molecular Biology Skin Tissue Inhibitor of Metalloproteinase-2 Proteolytic enzymes Proteins Cell Biology medicine.disease Molecular biology Carcinoma Basal Cell Carcinoma Squamous Cell Northern blot hybridization |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0022-202X |
| Popis: | Tumor cell invasion and metastasis are considered to represent a multistep process leading to the degradation of the extracellular matrix by proteolytic enzymes. The functional activity of matrix metalloproteinases (MMPs) is controlled by tissue inhibitor of metalloproteinases-2 (TINIP-2), which has been shown to inhibit tumor cell invasion and metastasis in vitro and in vivo . To assess the role of TIMP-2 in skin-derived epithelial tumors, we have analyzed the expression of TIMP-2 mRNA in primary tissue samples from human cutaneous basal (BCC) and squamous cell carcinoma (SCC) for a correlation with their different invasive and metastatic potential. Comparative quantitative analysis of TIMP-2 mRNA levels by Northern blot hybridization demonstrated significantly higher TIMP-2 tissue levels in BCC than in SCC, indicating an inverse correlation between TIMP-2 expression and the metastatic capacity of these tumors in vivo . By in situ hybridization, tumor stromal cells were identified as the principal source of TIMP-2 mRNA in both BCC and SCC. A comparable distribution has been reported previously for several matrix metalloproteinases in cutaneous BCC and SCC, indicating co-localization of metalloproteinases with their respective inhibitor. These results may suggest that TIMP-2 substantially contributes to the biological behavior of epithelium-derived skin tumors by significantly inhibiting tumor cell metastasis. |
| Databáze: | OpenAIRE |
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