In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis
| Autor: | Meivelu Moovendhan, Gnanakkan Ananthan, Arunachalam Palavesam, Jeganathan Arun, Ramamoorthy Sathishkumar, Sundararaj Selvakumar, Thirumalai Maruthiah |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antioxidant Polymers and Plastics DPPH Radical medicine.medical_treatment Size-exclusion chromatography Ascorbic Acid 02 engineering and technology Antioxidants 03 medical and health sciences chemistry.chemical_compound Spectroscopy Fourier Transform Infrared Materials Chemistry medicine Organic chemistry Monosaccharide Bacillaceae Phylogeny chemistry.chemical_classification Chromatography Superoxide Polysaccharides Bacterial Organic Chemistry Free Radical Scavengers 021001 nanoscience & nanotechnology Ascorbic acid 030104 developmental biology chemistry Galactose 0210 nano-technology |
| Zdroj: | Carbohydrate Polymers. 155:400-406 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2016.08.085 |
| Popis: | Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography. EPS extracted from the supernatant yielded a 56.1% total carbohydrate content. The ash and moisture content were 12.1% and 1.5% respectively. Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis. FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region. In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately. HMEPS showed higher reducing ability against superoxide radical and potency in chelating the ferrous ions. 10mg/ml of HMEPS was found equivalent to 2.7units of ascorbic acid through the total antioxidant assay. Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0. |
| Databáze: | OpenAIRE |
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