Progesterone Secretion and Proliferation in Cultured Rabbit Granulosa Cells under Conditions of β-D-Xyloside-Induced Inhibition of Proteoglycan Synthesis
| Autor: | Pierre Leymarie, J Bocquet, M Langris, A. Benhaim, C Feral |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Granulosa cell Chorionic Gonadotropin Glycosaminoglycan Internal medicine Cyclic AMP medicine Animals Secretion Glycosides Ovarian follicle Cells Cultured Progesterone Granulosa Cells biology Cell growth DNA Cell Biology General Medicine Progesterone secretion Endocrinology medicine.anatomical_structure Bucladesine Reproductive Medicine Proteoglycan biology.protein Female Proteoglycans Rabbits Follicle Stimulating Hormone Gonadotropin Cell Division |
| Zdroj: | Biology of Reproduction. 52:939-946 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod52.4.939 |
| Popis: | Proteoglycans present in follicular fluid are synthesized by granulosa cells under gonadotropin control. An inhibitor of proteoglycan synthesis, p-nitrophenyl-β-D-xyloside (β-D-xyloside) was used as a probe to study rabbit granulosa cell steroidogenesis and proliferation under abrogated proteoglycan synthesis. Granulosa cells isolated from rabbit preovulatory follicles were cultured 24 h in Minimum Essential Medium plus 2.5% fetal calf serum in the presence or absence of β-D-xyloside in the absence or presence of FSH inhibited proteoglycan synthesis and increased the amount of glycosaminoglycans (GAG). FSH-stimulated progesterone production was significantly correlated only with proteoglycan synthesis and not with GAG production. The addition of various concentrations of β-D-xyloside (0.1-4 mM) for 48 h to granulosa cells induced a dose-dependent inhibition of FSH-stimulated progesterone secretion and [ 3 H]thymidine incorporation into DNA. β-D-Xyloside concentrations lower than 1 mM induced an inhibition of FSH-stimulated progesterone secretion but had no significant effect of FSH-induced proliferation. One millimolar β-D-xyloside did not modify basal progesterone production, but in the presence of various doses (0.1-2.5 ng/ml) of FSH or hCG (0.1-1 IU/ml) it exerted a significant inhibitory effect on steroid secretion. Fifty percent inhibition was obtained for doses of FSH above 0.5 ng/ml. When cells were stimulated by db-cAMP instead of FSH, the inhibition of progesterone synthesis induced by 1 mM β-D-xyloside was slightly significant. This dose of β-D-xyloside markedly inhibited FSH-induced cAMP production. Thus, it seems that the major consequence of proteoglycan synthesis abrogation was at a step beyond cAMP synthesis. These results suggest a role of proteoglycan metabolism in maintaining important functions of granulosa cell steroidogenesis and proliferation |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|