Insulin regulation of glucokinase gene expression: Evidence against a role for sterol regulatory element binding protein 1 in primary hepatocytes

Autor: Jean Girard, Claudine Gregori, Jean-François Decaux, Isabelle Guillet-Deniau, Anne-Lise Pichard
Přispěvatelé: Génétique et pathologie moléculaires, Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Cochin (UMR_S567 / UMR 8104), Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Male
Transcription
Genetic
medicine.medical_treatment
Biochemistry
Sterol regulatory element binding protein
0302 clinical medicine
Structural Biology
Gene expression
Glucokinase
polycyclic compounds
Insulin
RNA
Small Interfering
Cells
Cultured
ComputingMilieux_MISCELLANEOUS
0303 health sciences
Gene knockdown
food and beverages
Small interfering RNA
3. Good health
Fatty acid synthase
lipids (amino acids
peptides
and proteins)
Sterol Regulatory Element Binding Protein 1
medicine.medical_specialty
Biophysics
Biology
digestive system
Gene Expression Regulation
Enzymologic
03 medical and health sciences
Internal medicine
Genetics
medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Gene Silencing
Rats
Wistar
Carbohydrate-responsive element-binding protein
Molecular Biology
030304 developmental biology
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Cell Biology
Sterol regulatory element-binding protein
Rats
Endocrinology
biology.protein
Hepatocytes
030217 neurology & neurosurgery
Zdroj: FEBS Letters
FEBS Letters, 2006, 580 (2), pp.410-414. ⟨10.1016/j.febslet.2005.12.032⟩
FEBS Letters, Wiley, 2006, 580 (2), pp.410-414. ⟨10.1016/j.febslet.2005.12.032⟩
ISSN: 0014-5793
1873-3468
Popis: Liver key genes for carbohydrate and lipid homeostasis are regulated by insulin and glucose. The sterol regulatory-element binding protein-1c (SREBP-1c) has emerged as a mediator of insulin effects on gene transcription, particularly on glucokinase (GK). In this paper, we show that despite stimulation of GK promoter transcription by overexpression of mature SREBP-1c, insulin induced GK transcription at least 4h ahead of accumulation of mature SREBP-1c in the nucleus. Importantly, the knockdown of SREBP-1 mRNA using a RNA-interference technique reduced the level of nuclear SREBP-1 protein, diminished fatty acid synthase mRNA level, but did not affect GK and L-pyruvate kinase mRNA levels. We concluded that SREBP-1 is unlikely to be the mediator of the early insulin effect on GK gene transcription.
Databáze: OpenAIRE
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