Methylation biomarkers for pleomorphic lobular breast cancer - a short report
Autor: | Cathy B. Moelans, Patrick W. B. Derksen, Horst Buerger, Gábor Cserni, Eva J. Vlug, Peter Bult, Paul J. van Diest, Cigdem Ercan |
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Jazyk: | angličtina |
Předmět: |
Pathology
Cancer Research Lobular breast cancer Cluster Analysis Promoter Regions Genetic skin and connective tissue diseases DNA METHYLATION Medicine(all) Women's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17] MAMMARY-CARCINOMA BRCA1 Protein Carcinoma Ductal Breast Nuclear Proteins General Medicine Methylation TUMORS DNA-Binding Proteins DIFFERENTIATION Oncology DNA methylation Molecular Medicine Epigenetics Female MutL Protein Homolog 1 EXPRESSION medicine.medical_specialty Breast Neoplasms DNA hypermethylation Biology Diagnosis Differential Breast cancer E-CADHERIN medicine Biomarkers Tumor Humans Breast carcinogenesis Pleomorphic lobular breast cancer neoplasms Adaptor Proteins Signal Transducing Original Paper Analysis of Variance Tumor Suppressor Proteins Tumor Protein p73 RASSF1A DNA Methylation medicine.disease BRCA1 Molecular medicine SPORADIC BREAST body regions Carcinoma Lobular Sporadic breast cancer Logistic Models ROC Curve Tumor progression PROMOTER HYPERMETHYLATION Cancer research Differential diagnosis MS-MLPA Multiplex Polymerase Chain Reaction |
Zdroj: | Analytical Cellular Pathology-Cellular Oncology, 38, 397-405 Cellular Oncology (Dordrecht) Cellular oncology, 38(5), 397. Springer Netherlands Analytical Cellular Pathology-Cellular Oncology, 38, 5, pp. 397-405 |
ISSN: | 2211-3428 2210-7177 |
DOI: | 10.1007/s13402-015-0241-9 |
Popis: | Contains fulltext : 152476.pdf (Publisher’s version ) (Open Access) BACKGROUND: Pleomorphic invasive lobular cancer (pleomorphic ILC) is a rare variant of ILC that is characterized by a classic ILC-like growth pattern combined with an infiltrative ductal cancer (IDC)-like high nuclear atypicality. There is an ongoing discussion whether pleomorphic ILC is a dedifferentiated form of ILC or in origin an IDC with a secondary loss of cohesion. Since gene promoter hypermethylation is an early event in breast carcinogenesis and thus may provide information on tumor progression, we set out to compare the methylation patterns of pleomorphic ILC, classic ILC and IDC. In addition, we aimed at analyzing the methylation status of pleomorphic ILC. METHODS: We performed promoter methylation profiling of 24 established and putative tumor suppressor genes by methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) analysis in 20 classical ILC, 16 pleomorphic ILC and 20 IDC cases. RESULTS: We found that pleomorphic ILC showed relatively low TP73 and MLH1 methylation levels and relatively high RASSF1A methylation levels compared to classic ILC. Compared to IDC, pleomorphic ILC showed relatively low MLH1 and BRCA1 methylation levels. Hierarchical cluster analysis revealed a similar methylation pattern for pleomorphic ILC and IDC, while the methylation pattern of classic ILC was different. CONCLUSION: This is the first report to identify TP73, RASSF1A, MLH1 and BRCA1 as possible biomarkers to distinguish pleomorphic ILC from classic ILC and IDC. |
Databáze: | OpenAIRE |
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