Methylation biomarkers for pleomorphic lobular breast cancer - a short report

Autor: Cathy B. Moelans, Patrick W. B. Derksen, Horst Buerger, Gábor Cserni, Eva J. Vlug, Peter Bult, Paul J. van Diest, Cigdem Ercan
Jazyk: angličtina
Předmět:
Pathology
Cancer Research
Lobular breast cancer
Cluster Analysis
Promoter Regions
Genetic
skin and connective tissue diseases
DNA METHYLATION
Medicine(all)
Women's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17]
MAMMARY-CARCINOMA
BRCA1 Protein
Carcinoma
Ductal
Breast
Nuclear Proteins
General Medicine
Methylation
TUMORS
DNA-Binding Proteins
DIFFERENTIATION
Oncology
DNA methylation
Molecular Medicine
Epigenetics
Female
MutL Protein Homolog 1
EXPRESSION
medicine.medical_specialty
Breast Neoplasms
DNA hypermethylation
Biology
Diagnosis
Differential
Breast cancer
E-CADHERIN
medicine
Biomarkers
Tumor
Humans
Breast carcinogenesis
Pleomorphic lobular breast cancer
neoplasms
Adaptor Proteins
Signal Transducing
Original Paper
Analysis of Variance
Tumor Suppressor Proteins
Tumor Protein p73
RASSF1A
DNA Methylation
medicine.disease
BRCA1
Molecular medicine
SPORADIC BREAST
body regions
Carcinoma
Lobular
Sporadic breast cancer
Logistic Models
ROC Curve
Tumor progression
PROMOTER HYPERMETHYLATION
Cancer research
Differential diagnosis
MS-MLPA
Multiplex Polymerase Chain Reaction
Zdroj: Analytical Cellular Pathology-Cellular Oncology, 38, 397-405
Cellular Oncology (Dordrecht)
Cellular oncology, 38(5), 397. Springer Netherlands
Analytical Cellular Pathology-Cellular Oncology, 38, 5, pp. 397-405
ISSN: 2211-3428
2210-7177
DOI: 10.1007/s13402-015-0241-9
Popis: Contains fulltext : 152476.pdf (Publisher’s version ) (Open Access) BACKGROUND: Pleomorphic invasive lobular cancer (pleomorphic ILC) is a rare variant of ILC that is characterized by a classic ILC-like growth pattern combined with an infiltrative ductal cancer (IDC)-like high nuclear atypicality. There is an ongoing discussion whether pleomorphic ILC is a dedifferentiated form of ILC or in origin an IDC with a secondary loss of cohesion. Since gene promoter hypermethylation is an early event in breast carcinogenesis and thus may provide information on tumor progression, we set out to compare the methylation patterns of pleomorphic ILC, classic ILC and IDC. In addition, we aimed at analyzing the methylation status of pleomorphic ILC. METHODS: We performed promoter methylation profiling of 24 established and putative tumor suppressor genes by methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) analysis in 20 classical ILC, 16 pleomorphic ILC and 20 IDC cases. RESULTS: We found that pleomorphic ILC showed relatively low TP73 and MLH1 methylation levels and relatively high RASSF1A methylation levels compared to classic ILC. Compared to IDC, pleomorphic ILC showed relatively low MLH1 and BRCA1 methylation levels. Hierarchical cluster analysis revealed a similar methylation pattern for pleomorphic ILC and IDC, while the methylation pattern of classic ILC was different. CONCLUSION: This is the first report to identify TP73, RASSF1A, MLH1 and BRCA1 as possible biomarkers to distinguish pleomorphic ILC from classic ILC and IDC.
Databáze: OpenAIRE
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