Investigation of antinociceptive, antipyretic, antiasthmatic, and spasmolytic activities of Brazilian Cannabis sativa L. roots in rodents
| Autor: | Larissa Araújo Rolim, Kátia Simoni Bezerra Lima, David Souza Silva, Tarcísio Cícero de Lima Araújo, Maria Helena Tavares de Matos, A.P.O. Monte, Janaine Almeida Neto, Fabrício Souza Silva, Pedro Modesto Nascimento Menezes, Mariana Coelho Brito, Emanuella Chiara Valença Pereira, Luciano Augusto de Araújo Ribeiro, Ricardo Lima |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Antipyretics Fever Cannabaceae Pain Pharmacology Plant Roots Mice 03 medical and health sciences 0302 clinical medicine In vivo Drug Discovery medicine Animals Eosinophilia Anti-Asthmatic Agents Antipyretic Rats Wistar Hot plate test Cannabis 030304 developmental biology Inflammation Analgesics 0303 health sciences Dose-Response Relationship Drug biology Plant Extracts Chemistry Parasympatholytics biology.organism_classification Rats Disease Models Animal Ovalbumin Nociception 030220 oncology & carcinogenesis biology.protein medicine.symptom Licking Brazil medicine.drug |
| Zdroj: | Journal of Ethnopharmacology. 278:114259 |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance Many studies are performed with the aerial parts of Cannabis sativa L. (Cannabaceae). However, roots remain poorly studied, despite citations in the scientific literature. The C. sativa roots are indicated for the treatment of pain, inflammation, fever, among other health problems. Aim of the study: This study aimed to evaluate the antinociceptive, antipyretic, antiasthmatic, and spasmolytic activities of C. sativa roots in experimental models using mice and rats. Material and methods The chemical composition of the aqueous extract of C. sativa roots (AECsR) was evaluated by LC-MS. The antinociceptive activity was assessed in mice by the induction of writhing with acetic acid, paw licking with formalin, and reactivity in the hot plate test. Fever was induced by the administration of a suspension of Saccharomyces cerevisiae in young rats. The asthmatic activity was performed with ovalbumin (OVA)-immunized mice with cellular and histological analysis. Finally, the spasmolytic activity was performed using mice isolated trachea. For in vivo studies, the doses were 12.5, 25, or 50 mg/kg whereas for in vitro, the concentration of AECsR was 729 μg/mL. Results From the LC-MS data, we identified p-coumaroyltyramine, feruloyltyramine canabissativine in AECsR. The extract promoted a reduction of writhing in all tested doses (12.5, 25, or 50 mg/kg). Similarly, it reduced the pain in the formalin test at doses of 12.5 and 50 mg/kg (first phase) and 12.5 and 25 mg/kg (second phase). In the hot plate test, the doses of 12.5, 25, and 50 mg/kg promoted antinociceptive effect at different times, and the lowest dose maintained its action in the analyzes performed at 60, 90, and 120 min after administration. The anti-inflammatory activity of AECsR was observed in the mouse model of asthma, reducing the total leukocyte count in the bronchoalveolar fluid (BALF) at a dose of 25 mg/kg, as well as reducing eosinophilia in all tested doses (12.5, 25, and 50 mg/kg). Histological analysis of lungs stained with H&E and PAS showed a reduction in the number of inflammatory cells in the perivascular and peribronchial region, as well as reduced mucus production. Conclusion The results suggest that AECsR promotes pain control, either by a central or inflammatory mechanism, and has antiasthmatic activity. However, there was no antipyretic or spasmolytic effect. |
| Databáze: | OpenAIRE |
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