TILs Immunophenotype in Breast Cancer Predicts Local Failure and Overall Survival: Analysis in a Large Radiotherapy Trial with Long-Term Follow-Up
Autor: | Ewan K.A. Millar, Lois Browne, Heather Ann Brauer, Sandra A O'Toole, Iveta Slapetova, Rachel Bradshaw, Fei Shang, Peter Graham, Julia Beretov, Yuqi Ren, Renee Whan |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty Multivariate analysis medicine.medical_treatment lcsh:RC254-282 Article 03 medical and health sciences 0302 clinical medicine Immunophenotyping Breast cancer breast cancer Internal medicine medicine tumour infiltrating lymphocytes (TILs) business.industry Cancer FOXP3 medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens immunophenotype Clinical trial Radiation therapy 030104 developmental biology 030220 oncology & carcinogenesis Cohort prognosis business |
Zdroj: | Cancers Volume 12 Issue 9 Cancers, Vol 12, Iss 2365, p 2365 (2020) |
ISSN: | 2072-6694 |
Popis: | Aim: To determine the prognostic significance of the immunophenotype of tumour-infiltrating lymphocytes (TILs) within a cohort of breast cancer patients with long-term follow-up. Methods: Multiplexed immunofluorescence and automated image analysis were used to assess the expression of CD3, CD8, CD20, CD68, Fox P3, PD-1 and PD-L1 in a clinical trial of local excision and radiotherapy randomised to a cavity boost or not (n = 485, median follow-up 16 years). Kaplan&ndash Meier and Cox multivariate analysis (MVA) methodology were used to ascertain relationships with local recurrence (LR), overall survival (OS) and disease-free survival (DFS). NanoString BC360 gene expression panel was applied to a subset of luminal patients to identify pathways associated with LR. Results: LR was predicted by low CD8 in MVA in the whole cohort (HR 2.34, CI 1.4&ndash 4.02, p = 0.002) and luminal tumours (HR 2.19, CI 1.23&ndash 3.92, p = 0.008) with associations with increased stromal components, decreased Tregs (FoxP3), inflammatory chemokines and SOX2. Poor OS was associated with low CD20 in the whole cohort (HR 1.73, CI 1.2&ndash 2.4, p = 0.002) and luminal tumours on MVA and low PD-L1 in triple-negative cancer (HR 3.44, CI 1.5&ndash 7, p = 0.003). Conclusions: Immunophenotype adds further prognostic data to help further stratify risk of LR and OS even in TILs low-luminal tumours. |
Databáze: | OpenAIRE |
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