5-Lipoxygenase Metabolite 4-HDHA Is a Mediator of the Antiangiogenic Effect of ω-3 Polyunsaturated Fatty Acids
| Autor: | Arianna Carughi, Kip M. Connor, C. M. Aderman, Elvira L. Liclican, Roberta J. Dennison, Molly R. Seaward, Yoshihide Kanaoka, Keirnan L. Willett, Lois E.H. Smith, Andreas Stahl, Karsten Gronert, John Paul SanGiovanni, Nathan M. Krah, Dalia Perelman, Przemyslaw Sapieha, Jing Chen |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Docosahexaenoic Acids Blotting Western Angiogenesis Inhibitors Article Mice Lipoxygenase Retinal Diseases Fatty Acids Omega-6 Internal medicine Fatty Acids Omega-3 medicine Animals Humans Cells Cultured Cell Proliferation Sprouting angiogenesis chemistry.chemical_classification Arachidonate 5-Lipoxygenase biology Reverse Transcriptase Polymerase Chain Reaction General Medicine Lipid signaling medicine.disease Immunohistochemistry Oxygen PPAR gamma Endocrinology chemistry Cyclooxygenase 2 Docosahexaenoic acid Arachidonate 5-lipoxygenase biology.protein lipids (amino acids peptides and proteins) Cyclooxygenase Retinopathy Polyunsaturated fatty acid |
| Zdroj: | Science Translational Medicine. 3 |
| ISSN: | 1946-6242 1946-6234 |
| Popis: | Lipid signaling is dysregulated in many diseases with vascular pathology, including cancer, diabetic retinopathy, retinopathy of prematurity, and age-related macular degeneration. We have previously demonstrated that diets enriched in ω-3 polyunsaturated fatty acids (PUFAs) effectively reduce pathological retinal neovascularization in a mouse model of oxygen-induced retinopathy, in part through metabolic products that suppress microglial-derived tumor necrosis factor–α. To better understand the protective effects of ω-3 PUFAs, we examined the relative importance of major lipid metabolic pathways and their products in contributing to this effect. ω-3 PUFA diets were fed to four lines of mice deficient in each key lipid-processing enzyme (cyclooxygenase 1 or 2, or lipoxygenase 5 or 12/15), retinopathy was induced by oxygen exposure; only loss of 5-lipoxygenase (5-LOX) abrogated the protection against retinopathy of dietary ω-3 PUFAs. This protective effect was due to 5-LOX oxidation of the ω-3 PUFA lipid docosahexaenoic acid to 4-hydroxy-docosahexaenoic acid (4-HDHA). 4-HDHA directly inhibited endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator–activated receptor γ (PPARγ), independent of 4-HDHA’s anti-inflammatory effects. Our study suggests that ω-3 PUFAs may be profitably used as an alternative or supplement to current anti–vascular endothelial growth factor (VEGF) treatment for proliferative retinopathy and points to the therapeutic potential of ω-3 PUFAs and metabolites in other diseases of vasoproliferation. It also suggests that cyclooxygenase inhibitors such as aspirin and ibuprofen (but not lipoxygenase inhibitors such as zileuton) might be used without losing the beneficial effect of dietary ω-3 PUFA. |
| Databáze: | OpenAIRE |
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