Fluorescent nanodiamonds engage innate immune effector cells: A potential vehicle for targeted anti-tumor immunotherapy
| Autor: | William E. Carson, Lorena P. Suarez-Kelly, Min S. Wang, Isaac Rampersaud, Susheela Tridandapani, Lianbo Yu, Amanda Campbell, Jonathan P. Butchar, Arfaan A. Rampersaud, Ambika Bumb |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class medicine.medical_treatment Cell Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Monoclonal antibody Monocytes Article Nanodiamonds Mice 03 medical and health sciences Immune system Adjuvants Immunologic Neoplasms medicine Animals Humans General Materials Science Cells Cultured Fluorescent Dyes Immunity Cellular Mice Inbred BALB C Innate immune system biology Immunotherapy 021001 nanoscience & nanotechnology Immunity Innate Cell biology Killer Cells Natural RAW 264.7 Cells 030104 developmental biology medicine.anatomical_structure Cytokine Immunology biology.protein Molecular Medicine Tumor necrosis factor alpha Antibody 0210 nano-technology |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 13:909-920 |
| ISSN: | 1549-9634 |
| Popis: | Fluorescent nanodiamonds (FNDs) are nontoxic, infinitely photostable, and emit fluorescence in the near infrared region. Natural killer (NK) cells and monocytes are part of the innate immune system and are crucial to the control of carcinogenesis. FND-mediated stimulation of these cells may serve as a strategy to enhance anti-tumor activity. FNDs were fabricated with a diameter of 70±28 nm. Innate immune cell FND uptake, viability, surface marker expression, and cytokine production were evaluated in vitro. Evaluation of fluorescence emission from the FNDs was conducted in an animal model. In vitro results demonstrated that treatment of immune cells with FNDs resulted in significant dose-dependent FND uptake, no compromise in cell viability, and immune cell activation. FNDs were visualized in an animal model. Hence, FNDs may serve as novel agents with “track and trace” capabilities to stimulate innate immune cell anti-tumor responses, especially as FNDs are amenable to surface-conjugation with immunomodulatory molecules. |
| Databáze: | OpenAIRE |
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