Monorail/Foxa2 regulates floorplate differentiation and specification of oligodendrocytes, serotonergic raphé neurones and cranial motoneurones
| Autor: | M Rees, Gerd-Jörg Rauch, William H. J. Norton, Uwe Strähle, Hiroki Teraoka, Brianne Diamond, Hans-Martin Pogoda, Robert Geisler, Zsolt Lele, Carl-Philipp Heisenberg, Thomas F. Schilling, Sepand Rastegar, R M Gardiner, S Mercurio, Stephen W. Wilson, H G Frohnhoefer, Claire Russell, Corinne Houart, Carl J. Neumann, William S. Talbot, Heather L. Stickney, M Mangoli |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Norton, W, Mangoli, M, Lele, Z, Pogoda, H, Diamond, B, Mercurio, S, Russell, C, Teraoka, H, Stickney, H, Rauch, G, Heisenberg, C, Houart, C, Schilling, T, Frohnhoefer, H, Rastegar, S, Neumann, C, Gardiner, R, Strähle, U, Geisler, R, Rees, M, Talbot, W, Wilson, S, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Central Nervous System
Embryo Nonmammalian Transcription Factor MESH: Embryonic Induction Trochlear Nerve MESH: Gene Expression Regulation Developmental MESH: Animals Zebrafish reproductive and urinary physiology Motor Neurons Embryonic Induction 0303 health sciences 030302 biochemistry & molecular biology Gene Expression Regulation Developmental MESH: Raphe Nuclei Forkhead Transcription Factors MESH: Oligodendroglia MESH: Transcription Factors respiratory system Cell biology Neuroepithelial cell Oligodendroglia medicine.anatomical_structure Trans-Activator Zebrafish Protein embryonic structures Anatomy Hedgehog Protein MESH: Motor Neurons medicine.medical_specialty Cell type Serotonin animal structures MESH: Mutation MESH: Trans-Activators Central nervous system Hindbrain MESH: Zebrafish Proteins Biology Motor Neuron Article Midbrain 03 medical and health sciences MESH: Forkhead Transcription Factors Internal medicine MESH: Trochlear Nerve medicine Animals MESH: Central Nervous System Hedgehog Proteins MESH: Zebrafish Molecular Biology 030304 developmental biology Raphe Animal Neural tube MESH: Embryo Nonmammalian [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Midline development Forkhead Transcription Factor Cell Biology MESH: Hedgehog Proteins Zebrafish Proteins Endocrinology nervous system Hedgehog signalling Mutation Trans-Activators Raphe Nuclei Axon guidance MESH: Serotonin Developmental Biology Transcription Factors |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2005, 132 (4), pp.645-58. ⟨10.1242/dev.01611⟩ |
| ISSN: | 0950-1991 1477-9129 |
| DOI: | 10.1242/dev.01611⟩ |
| Popis: | In this study, we elucidate the roles of the winged-helix transcription factor Foxa2 in ventral CNS development in zebrafish. Through cloning of monorail (mol), which we find encodes the transcription factor Foxa2, and phenotypic analysis of mol-/- embryos,we show that floorplate is induced in the absence of Foxa2 function but fails to further differentiate. In mol-/- mutants, expression of Foxa and Hh family genes is not maintained in floorplate cells and lateral expansion of the floorplate fails to occur. Our results suggest that this is due to defects both in the regulation of Hh activity in medial floorplate cells as well as cell-autonomous requirements for Foxa2 in the prospective laterally positioned floorplate cells themselves.Foxa2 is also required for induction and/or patterning of several distinct cell types in the ventral CNS. Serotonergic neurones of the raphénucleus and the trochlear motor nucleus are absent in mol-/- embryos, and oculomotor and facial motoneurones ectopically occupy ventral CNS midline positions in the midbrain and hindbrain. There is also a severe reduction of prospective oligodendrocytes in the midbrain and hindbrain. Finally, in the absence of Foxa2, at least two likely Hh pathway target genes are ectopically expressed in more dorsal regions of the midbrain and hindbrain ventricular neuroepithelium, raising the possibility that Foxa2 activity may normally be required to limit the range of action of secreted Hh proteins. |
| Databáze: | OpenAIRE |
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