A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column
| Autor: | Koichi Harayama, Akira Toriba, Yasushi Nagatomi, Masayoshi Tamura, Naoki Mochizuki, Kazuichi Hayakawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Health
Toxicology and Mutagenesis lcsh:Medicine Food Contamination LC-Orbitrap MS Toxicology Quechers Orbitrap Zea mays Diacetoxyscirpenol Article Mass Spectrometry law.invention Matrix (chemical analysis) chemistry.chemical_compound Fusarium Japan law Fumonisin simultaneous determination Zearalenone Triticum Fumonisin B2 cereals Fumonisin B1 Chromatography lcsh:R Hordeum Mycotoxins chemistry Fusarium toxins pentafluorophenyl column Edible Grain Chromatography Liquid Environmental Monitoring |
| Zdroj: | Toxins, Vol 7, Iss 5, Pp 1664-1682 (2015) Toxins Volume 7 Issue 5 Pages 1664-1682 |
| ISSN: | 2072-6651 |
| Popis: | A high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) method was developed for simultaneous determination of 20 Fusarium toxins (nivalenol, fusarenon-X, deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, HT-2 toxin, T-2 toxin, neosolaniol, diacetoxyscirpenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisin A1, fumonisin A2, fumonisin A3, zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol) in cereals. The separation of 20 Fusarium toxins with good peak shapes was achieved using a pentafluorophenyl column, and Orbitrap MS was able to detect accurately from cereal matrix components within ±0.77 ppm. The samples were prepared using a QuEChERS kit for extraction and a multifunctional cartridge for purification. The linearity, repeatability, and recovery of the method were > 0.9964, 0.8%–14.7%, and 71%–106%, respectively. Using this method, an analysis of 34 commercially available cereals detected the presence of deoxynivalenol, 15-acetyl deoxynivalenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisn A1, fumonisin A2, fumonisin A3, and zearalenone in corn samples with high concentration and frequency. Trichothecenes was detected from wheat samples with high frequency in particular, the concentration of deoxynivalenol was high. Conversely, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples. |
| Databáze: | OpenAIRE |
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