Streamlined Synthesis and Assembly of a Hybrid Sensing Architecture with Solid Binding Proteins and Click Chemistry
| Autor: | François Baneyx, Cole A. DeForest, Brian J. F. Swift, Jared A. Shadish |
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| Rok vydání: | 2017 |
| Předmět: |
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Molecular Nanostructure Surface Properties Aptamer Green Fluorescent Proteins Sequence (biology) Nanotechnology 02 engineering and technology Sulfides Protein Engineering 010402 general chemistry 01 natural sciences Biochemistry Article Catalysis chemistry.chemical_compound Colloid and Surface Chemistry Particle Size Manganese Binding Sites DNA General Chemistry Silicon Dioxide 021001 nanoscience & nanotechnology Combinatorial chemistry Zinc sulfide Cycloaddition 0104 chemical sciences Chloramphenicol chemistry Zinc Compounds Click chemistry Click Chemistry Bioorthogonal chemistry 0210 nano-technology Hybrid material |
| Zdroj: | J Am Chem Soc |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Combining bioorthogonal chemistry with the use of proteins engineered with adhesive and morphogenetic solid-binding peptides is a promising route for synthesizing hybrid materials with the economy and efficiency of living systems. Using optical sensing of chloramphenicol as a proof of concept, we show here that a GFP variant engineered with zinc sulfide and silica-binding peptides on opposite sides of its β-barrel supports the fabrication of protein-capped ZnS:Mn nanocrystals that exhibit the combined emission signatures of organic and inorganic fluorophores. Conjugation of a chloramphenicol-specific DNA aptamer to the protein shell through strain-promoted azide–alkyne cycloaddition and spontaneous concentration of the resulting nanostructures onto SiO(2) particles mediated by the silica-binding sequence enables visual detection of environmentally and clinically relevant concentrations of chloramphenicol through analyte-mediated inner filtering of sub-330 nm excitation light. |
| Databáze: | OpenAIRE |
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