Primary microRNA transcripts are processed co-transcriptionally
| Autor: | Morlando, Mariangela, Ballarino, Monica, Gromak, N, Pagano, Francesca, Bozzoni, Irene, Proudfoot, Nj |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Ribonuclease III
Chromatin Immunoprecipitation Transcription Genetic RNA Splicing RNA polymerase II beta-Globins Article Microprocessor complex 03 medical and health sciences 0302 clinical medicine Genetic Structural Biology Transcription (biology) microRNA Humans Molecular Biology Drosha 030304 developmental biology Genetics 0303 health sciences biology Intron Chromatin HeLa Cells Introns MicroRNAs Microtubule-Associated Proteins Nucleic Acid Conformation RNA Polymerase II Chromatin Immunoprecipitation Chromatin genetics/metabolism HeLa Cells Humans Introns MicroRNAs chemistry/genetics/metabolism Microtubule-Associated Proteins genetics Nucleic Acid Conformation RNA Polymerase II genetics/metabolism RNA Splicing Ribonuclease III genetics/metabolism Transcription Genetic beta-Globins genetics RNA splicing biology.protein Transcription 030217 neurology & neurosurgery Dicer |
| Zdroj: | Scopus-Elsevier CIÊNCIAVITAE Nature structural biology (2008). info:cnr-pdr/source/autori:Morlando M., Ballarino M., Gromak N., Pagano F., Bozzoni I. and Proudfoot N./titolo:Primary microRNA transcripts are processed co-transcriptionally,/doi:/rivista:Nature structural biology/anno:2008/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| ISSN: | 1545-9985 1545-9993 |
| Popis: | MicroRNAs are processed by Drosha, and previous data had suggested that this occurred soon after transcription. Following Drosha recruitment and using nuclear run-on technology, data now indicate that miRNAs both within and outside of introns are processed co-transcriptionally and that exonucleases enter the fray to aid processing before splicing, implying that Drosha cleavage influences the maturation of the pre-mRNAs in which miRNAs reside. microRNAs (miRNAs) are generated from long primary (pri-) RNA polymerase II (Pol II)–derived transcripts by two RNase III processing reactions: Drosha cleavage of nuclear pri-miRNAs and Dicer cleavage of cytoplasmic pre-miRNAs. Here we show that Drosha cleavage occurs during transcription acting on both independently transcribed and intron-encoded miRNAs. We also show that both 5′-3′ and 3′-5′ exonucleases associate with the sites where co-transcriptional Drosha cleavage occurs, promoting intron degradation before splicing. We finally demonstrate that miRNAs can also derive from 3` flanking transcripts of Pol II genes. Our results demonstrate that multiple miRNA-containing transcripts are co-transcriptionally cleaved during their synthesis and suggest that exonucleolytic degradation from Drosha cleavage sites in pre-mRNAs may influence the splicing and maturation of numerous mRNAs. |
| Databáze: | OpenAIRE |
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