Analysis of long non-coding RNA (lncRNA) expression in hepatitis B patients
| Autor: | Aycan Asik, Besra Ozmen Yelken, Süheyla Serin Senger, Tugce Balci Okcanoglu, Sukran Kose, Cagla Kayabasi, Didem Çelik, Cigir Biray Avci, Sunde Yilmaz Susluer, Cumhur Gündüz |
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| Přispěvatelé: | Ege Üniversitesi |
| Rok vydání: | 2018 |
| Předmět: |
resolved hepatitis B
Adult Gene Expression Regulation Viral Male 0301 basic medicine Hepatitis B virus HBsAg Small interfering RNA inactive HBsAg carrier medicine.disease_cause Epigenesis Genetic Hepatitis B Antigens Young Adult 03 medical and health sciences lncRNA Hepatitis B Chronic Gene expression HBV Humans Gene silencing Medicine chronic hepatitis B Gene Silencing RNA Small Interfering Aged Regulation of gene expression lcsh:R5-920 business.industry Liver Neoplasms General Medicine Middle Aged Prognosis Healthy Volunteers Long non-coding RNA 030104 developmental biology HBeAg Cancer research Female RNA Long Noncoding lcsh:Medicine (General) business Research Article |
| Zdroj: | Bosnian Journal of Basic Medical Sciences, Vol 18, Iss 2 (2018) |
| ISSN: | 1840-4812 1512-8601 2966-9510 |
| DOI: | 10.17305/bjbms.2018.2800 |
| Popis: | WOS: 000433285000006 PubMed ID: 29669510 Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes, including epigenetic regulation, cell-cycle control, and transcriptional/translational regulation of gene expression. Differential expression of lncRNAs and disruption of the regulatory processes are recognized as critical steps in cancer development. The role of lncRNAs in hepatitis B virus (HBV) infection is not well understood. Here we analyzed the expression of 135 lncRNAs in plasma samples of 82 HBV patients (classified as chronic patients, inactive carriers, or resolved patients) at diagnosis and at 12 months of treatment in relation to control group (81 healthy volunteers). We also investigated the effect of small interfering RNA (siRNA)-mediated silencing of lincRNA-SFMBT2 on HBV-positive human liver cancer cell line. lncRNA expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Chemically synthesized siRNAs were transfected into the cell lines using Lipofectamine 2000 Reagent (Thermo Fisher Scientific). HBV DNA and HBsAg and HBeAg were detected in transfected cultures by real-time PCR and ELISA, respectively, using commercial kits. We observed changes in lncRNA expression in all three HBV groups, compared to control group. Most notably, the expression of anti-NOS2A, lincRNA-SFMBT2, and Zfhx2as was significantly increased and expression of Y5 lncRNA was decreased in chronic HBV patients. A decreased Y5 expression and increased lincRNA-SFMBT2 expression were observed in inactive HBsAg carriers. The expression of HOTTIP, MEG9, and PCAT-32 was increased in resolved HBV patients, and no significant change in the expression of Y5 was observed, compared to control group. siRNA-mediated inhibition of lincRNA-SFMBT2 decreased the level of HBV DNA in human liver cancer cells. Further research is needed to confirm the prognostic as well as therapeutic role of these lncRNAs in HBV patients. TUBITAK (the Scientific and Technological Research Council of Turkey) grantTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215S225] This work was supported by TUBITAK (the Scientific and Technological Research Council of Turkey) grant (Project No: 215S225). |
| Databáze: | OpenAIRE |
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