Molecular Analysis of nuc-1+, a Gene Controlling Phosphorus Acquisition in Neurospora crassa
| Autor: | Seogchan Kang, Metzenberg, R. L. |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Base Sequence
Genotype Neurospora crassa Transcription Genetic Genetic Linkage Genes Fungal Genetic Complementation Test Molecular Sequence Data Restriction Mapping Phosphorus Saccharomyces cerevisiae Cell Biology Alkaline Phosphatase Blotting Southern fluids and secretions Sequence Homology Nucleic Acid Mutation Amino Acid Sequence Chromosomes Fungal Cloning Molecular DNA Fungal Molecular Biology Crosses Genetic Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.10.11.5839-5848.1990 |
| Popis: | In response to phosphorus starvation, Neurospora crassa makes several enzymes that are undetectable or barely detectable in phosphate-sufficient cultures. The nuc-1+ gene, whose product regulates the synthesis of these enzymes, was cloned and sequenced. The nuc-1+ gene encodes a protein of 824 amino acids with a predicted molecular weight of 87,429. The amino acid sequence shows homology with two yeast proteins whose functions are analogous to that of the NUC-1 protein. Two nuc-1+ transcripts of 3.2 and 3.0 kilobases were detected; they were present in similar amounts during growth at low or high phosphate concentrations. The nuc-2+ gene encodes a product normally required for NUC-1 function, and yet a nuc-2 mutation can be complemented by overexpression of the nuc-1+ gene. This implies physical interactions between NUC-1 protein and the negative regulatory factor(s) PREG and/or PGOV. Analysis of nuc-2 and nuc-1; nuc-2 strains transformed by the nuc-1+ gene suggests that phosphate directly affects the level or activity of the negative regulatory factor(s) controlling phosphorus acquisition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|