Ultrastructural studies of the mode of penetration by Phoma macdonaldiiin sunflower seedlings
| Autor: | Ali Mohammad Roustaee, G. Barrault, Grégory Dechamp-Guillaume, Robert Dargent, Brigitte Gelie, Colette Savy |
|---|---|
| Přispěvatelé: | Laboratoire de Génie Informatique et d'Automatique de l'Artois (LGI2A), Université d'Artois (UA), Unité des Sciences du Sol, Institut National de la Recherche Agronomique (INRA), Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE) |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
0106 biological sciences
Black stem Hypha Interactions Germ tube Plant Science 01 natural sciences Conidium subcuticular hyphae 03 medical and health sciences Helianthus annuus Botany 030304 developmental biology 0303 health sciences Appressorium biology fungi biology.organism_classification Phytopathologie et phytopharmacie [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Germination Phoma Ultrastructure Agronomy and Crop Science 010606 plant biology & botany host–pathogen |
| Zdroj: | Phytopathology Phytopathology, American Phytopathological Society, 2000, 90 (8), pp.915-920. ⟨10.1094/PHYTO.2000.90.8.915⟩ |
| ISSN: | 0031-949X |
| DOI: | 10.1094/PHYTO.2000.90.8.915⟩ |
| Popis: | International audience; An ultrastructural investigation of the artificial inoculation of sunflower with Phoma macdonaldii conidia was undertaken using light, scanning, and transmission electron microscopy to elucidate the host-parasite relationship. The behavior of the conidia deposited on the cotyledon petiole was investigated at various time intervals after inoculation. Conidia adhesion and germination were observed first. The cotyledon petiole was invaded by the fungus directly through the cuticle and via stomata. Externally, the spore and germ tube were covered with a mucilaginous polysaccharide sheath of a cotton-like appearance and of variable thickness. At the time of penetration, the host cuticle was perforated mechanically. The cuticle was slightly depressed and no enzymatic alteration could be observed. The fungus did not form appressoria on the surface of the host tissues but developed an infection peg. As soon as the cuticle barrier was crossed, the fungus rapidly colonized the host parietal layer. In a first step, the plasmalemma of the host cell appeared to be stuck against the cell wall. As soon as the fungus passed through the epidermal cell wall to reach the host cytoplasm, the plasmalemma was disrupted, and the subsequent rapid breakdown of cell integrity favored the colonization of the tissues by the pathogen. |
| Databáze: | OpenAIRE |
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