Expression of Rta in B Lymphocytes during Epstein-Barr Virus Latency
| Autor: | Lin-Chen Huang, Sseu-Pei Hwang, Wen-Hung Wang, Min-Hsuan Lin, Chung-Wen Kuo, Hsiang-Hung Huang, Li-Kwan Chang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Gene Expression Regulation
Viral Epstein-Barr Virus Infections Herpesvirus 4 Human Nucleolus viruses Biology medicine.disease_cause Virus Cell Line Immediate-Early Proteins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Structural Biology Transcription (biology) hemic and lymphatic diseases RNA polymerase medicine Humans Molecular Biology Gene 030304 developmental biology 0303 health sciences Messenger RNA B-Lymphocytes RNA-Binding Proteins biochemical phenomena metabolism and nutrition Epstein–Barr virus Molecular biology Virus Latency HEK293 Cells chemistry Lytic cycle Host-Pathogen Interactions Trans-Activators 030217 neurology & neurosurgery |
| Zdroj: | Journal of molecular biology. 432(19) |
| ISSN: | 1089-8638 |
| Popis: | Rta of Epstein-Barr virus (EBV) is thought to be expressed only during the lytic cycle to promote the transcription of lytic genes. However, we found that Rta is expressed in EBV-infected B cells during viral latency, at levels detectable by immunoblot analysis. Latent Rta expression cannot be attributed to spontaneous lytic activation, as we observed that more than 90% of Akata, P3HR1, and 721 cells latently infected by EBV express Rta. We further found that Rta is sequestered in the nucleolus during EBV latency through its interaction with MCRS2, a nucleolar protein. When Rta is sequestered in the nucleolus, it no longer activates RNA polymerase II-driven transcription, thus explaining why Rta expression during latency does not transactivate EBV lytic genes. Additional experiments showed that Rta can bind to 18S rRNA and become incorporated into ribosomes, and a transient transfection experiment showed that Rta promotes translation from an mRNA reporter. These findings reveal that Rta has novel functions beyond transcriptional activation during EBV latency and may have interesting implications for the concept of EBV latency. |
| Databáze: | OpenAIRE |
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