Targeted inhibition of the serotonin 5HT2A receptor improves coronary patency in an in vivo model of recurrent thrombosis
Autor: | Andrew L. Frelinger, Karin Przyklenk, Hussien A. Al-Shamma, Michael Morgan, Matthew D. Linden, YouFu Li, Marc R. Barnard, John W. Adams, Alan D. Michelson, Peter Whittaker |
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Rok vydání: | 2010 |
Předmět: |
Blood Platelets
Time Factors Drug Inverse Agonism Platelet Aggregation 5-HT2A receptor Morpholines Hemorrhage Pharmacology Article Dogs P2Y12 Fibrinolytic Agents Recurrence Coronary Circulation Serotonin 5-HT2 Receptor Antagonists Animals Medicine Receptor Serotonin 5-HT2A Platelet Serotonin Antagonists Receptor Vascular Patency 5-HT receptor business.industry Coronary Thrombosis Hemodynamics Hematology Disease Models Animal Benzamides Pyrazoles Platelet aggregation inhibitor business Platelet Aggregation Inhibitors |
Zdroj: | Journal of Thrombosis and Haemostasis. 8:331-340 |
ISSN: | 1538-7836 |
Popis: | Release of serotonin and activation of serotonin 5HT2A receptors on platelet surfaces is a potent augmentative stimulus for platelet aggregation. However, earlier-generation serotonin receptor antagonists were not successfully exploited as antiplatelet agents, possibly owing to their lack of specificity for the 5HT2A receptor subtype.To assess whether targeted inhibition of the serotonin 5HT2A receptor attenuates recurrent thrombosis and improves coronary patency in an in vivo canine model mimicking unstable angina.In protocol 1, anesthetized dogs were pretreated with a novel, selective inverse agonist of the 5HT2A receptor (APD791) or saline. Recurrent coronary thrombosis was then initiated by coronary artery injury+stenosis, and coronary patency was monitored for 3 h. Protocol 2 was similar, except that: (i) treatment with APD791 or saline was begun 1 h after the onset of recurrent thrombosis; (ii) template bleeding time was measured; and (iii) blood samples were obtained for in vitro flow cytometric assessment of platelet responsiveness to serotonin.APD791 attenuated recurrent thrombosis, irrespective of the time of treatment: in both protocols, flow-time area (index of coronary patency; normalized to baseline coronary flow) averaged 58-59% (P0.01) following administration of APD791 vs. 21-28% in saline controls. Moreover, the in vivo antithrombotic effect of APD791 was not accompanied by increased bleeding, but was associated with significant and selective inhibition of serotonin-mediated platelet activation.5HT2A receptor inhibition with APD791, even when initiated after the onset of recurrent thrombosis, improves coronary patency in the in vivo canine model. |
Databáze: | OpenAIRE |
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