Prefabricated 3D-Printed Tissue-Engineered Bone for Mandibular Reconstruction
| Autor: | Yuan-Ming Geng, Kausik Kapat, Shang-Bin Liu, Qian Li, Shuyi Li, Shuai-Shuai Cao, Yue-Juan Che, Pedro Miranda, Wu Gang, Fidel Hugo Perera, Hendrik Terheyden, Miao Zhou |
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| Přispěvatelé: | Oral Implantology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Primates
Scaffold 3d printed Materials science bone graft Biomedical Engineering Mandible Bone morphogenetic protein Article Biomaterials chemistry.chemical_compound In vivo Positron Emission Tomography Computed Tomography prefabrication Animals Mandibular reconstruction Bone regeneration Tissue Scaffolds Latissimus dorsi muscle 3D printing PLGA/TCP PLGA chemistry Printing Three-Dimensional Mandibular Reconstruction TCP Biomedical engineering |
| Zdroj: | Cao, S-S, Li, S-Y, Geng, Y-M, Kapat, K, Liu, S-B, Perera, F H, Li, Q, Terheyden, H, Wu, G, Che, Y-J, Miranda, P & Zhou, M 2021, ' Prefabricated 3D-Printed Tissue-Engineered Bone for Mandibular Reconstruction : A Preclinical Translational Study in Primate ', ACS Biomaterials Science and Engineering, vol. 7, no. 12, pp. 5727-5738 . https://doi.org/10.1021/acsbiomaterials.1c00509 ACS Biomaterials Science and Engineering, 7(12), 5727-5738. American Chemical Society ACS Biomaterials Science & Engineering |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.1c00509 |
| Popis: | © 2021 American Chemical Society.The advent of three dimensionally (3D) printed customized bone grafts using different biomaterials has enabled repairs of complex bone defects in various in vivo models. However, studies related to their clinical translations are truly limited. Herein, 3D printed poly(lactic-co-glycolic acid)/β-tricalcium phosphate (PLGA/TCP) and TCP scaffolds with or without recombinant bone morphogenetic protein -2 (rhBMP-2) coating were utilized to repair primate's large-volume mandibular defects and compared efficacy of prefabricated tissue-engineered bone (PTEB) over direct implantation (without prefabrication). 18F-FDG PET/CT was explored for real-time monitoring of bone regeneration and vascularization. After 3-month's prefabrication, the original 3D-architecture of the PLGA/TCP-BMP scaffold was found to be completely lost, while it was properly maintained in TCP-BMP scaffolds. Besides, there was a remarkable decrease in the PLGA/TCP-BMP scaffold density and increase in TCP-BMP scaffolds density during ectopic (within latissimus dorsi muscle) and orthotopic (within mandibular defect) implantation, indicating regular bone formation with TCP-BMP scaffolds. Notably, PTEB based on TCP-BMP scaffold was successfully fabricated with pronounced effects on bone regeneration and vascularization based on radiographic, 18F-FDG PET/CT, and histological evaluation, suggesting a promising approach toward clinical translation. |
| Databáze: | OpenAIRE |
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