Isolation and Identification of Cytotoxic Compounds from Aeschynomene fascicularis, a Mayan Medicinal Plant
| Autor: | Luis W. Torres-Tapia, Sergio R. Peraza-Sánchez, Edgar Caamal-Fuentes, Rosa Moo-Puc |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
antiproliferative activity
Ethyl acetate Pharmaceutical Science Antineoplastic Agents Biology Plant Roots Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry Drug Discovery Cytotoxic T cell Humans MTT assay Physical and Theoretical Chemistry Cytotoxicity Dichloromethane Cell Proliferation cytotoxic activity Plants Medicinal Traditional medicine Cytotoxins Organic Chemistry Fabaceae Hep G2 Cells chemistry Chemistry (miscellaneous) Cell culture visual_art flavonoids visual_art.visual_art_medium Plant Bark Mayan medicinal plant Molecular Medicine Bark Drug Screening Assays Antitumor HeLa Cells |
| Zdroj: | Molecules Volume 20 Issue 8 Pages 13563-13574 Molecules, Vol 20, Iss 8, Pp 13563-13574 (2015) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules200813563 |
| Popis: | The plant Aeschynomene fascicularis (Fabaceae) has been used in Mayan traditional medicine in the Yucatan peninsula. However, the compounds present in the plant responsible for its curative properties have not yet been investigated. Aeschynomene fascicularis root bark was extracted with 100% methanol to obtain a crude extract. The methanol extract was partitioned successively with solvents with increasing polarity to obtain the corresponding hexane (Hx), dichloromethane (DCM) and ethyl acetate fractions (EtOAc), as well as a residual water-alcoholic fraction. These fractions were tested for their cytotoxic activities using an MTT assay against Hep-2 cancer cell lines. The Hx fraction led to the isolation of spinochalcone C (1), spinochalcone A (2), isocordoin (3) and secundiflorol G (4). Their structures were identified based on spectroscopic evidence and chemical properties. All compounds were subjected to cytotoxicity and antiproliferative assays against a panel of seven cell lines, including one normal-type cell line. Spinochalcone A (2) exhibited cytotoxic activity against DU-145 cell line and antiproliferative activity against the KB cell line. Secundiflorol G (4) showed strong cytotoxic activity towards KB and Hep-2 cell lines. In addition, isocordoin (3) showed moderate activity on KB, Hep-2 and DU-145 cell lines. The active Compounds 2, 3 and 4 are potential therapeutic entities against cancer. |
| Databáze: | OpenAIRE |
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