Motility and fertility of bull sperm in whole milk extender containing antioxidants
| Autor: | Michael T. Kaproth, Robert H. Foote, C.C. Brockett |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Glycerol
Male Antioxidant Taurine medicine.medical_treatment Hypotaurine Semen Ascorbic Acid Antioxidants law.invention Cyclic N-Oxides Superoxide dismutase chemistry.chemical_compound Endocrinology Food Animals law Casein medicine Animals Food science Cryopreservation biology Superoxide Dismutase urogenital system Extender General Medicine Ascorbic acid Egg Yolk Glutathione Spermatozoa Sperm Fertility Milk chemistry Biochemistry Sperm Motility biology.protein Cattle Spin Labels Animal Science and Zoology Reactive Oxygen Species Oxidation-Reduction Semen Preservation |
| Zdroj: | Animal Reproduction Science. 71:13-23 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/s0378-4320(02)00018-0 |
| Popis: | Bull sperm are exposed to aerobic conditions during processing before freezing, and they have little endogenous antioxidant to protect them against reactive oxygen species that may be present. Seventeen laboratory studies and two field trials were conducted with 174 semen collections from bulls in an artificial breeding cooperative. More than 250 combinations and concentrations of reduced glutathione (GSH), superoxide dismutase (SOD), ascorbic acid, hypotaurine (HPT), 2,2,6,6-tetramethylpeperidine-1-oxyl (Tempo) and 4-hydroxy-2, 2, 6, 6-tetramethylpeperidine (Tempol) were tested by adding these compounds to fresh semen, and to a whole milk (WM) glycerol extender. Semen packaged in straws in the WM extender was frozen with liquid nitrogen. The motility of frozen-thawed sperm during storage at 25 or 5 degrees C after freezing was compared with semen stored without freezing. Antioxidants generally were not beneficial, except the percentage of motile sperm was improved by 6-11% units (P0.05) when sperm were stored unfrozen or after freezing when 0.5mM of GSH with or without SOD was added. In two field trials, non-return rates were 71.9, 69.5 and 70.9% (P0.05) with WM containing 0.0, 0.5 and 1.0mM of GSH, respectively, and 74.0 and 73.9% with WM and WM plus 0.5mM of GSH and 100U/ml of SOD (P0.05). WM contains an abundant supply of casein which is an antioxidant, and additional antioxidants were ineffective in improving motility of sperm immediately after freezing and thawing or in affecting fertility. However, sperm responses were different in egg yolk-Tris extender. Sperm in this egg yolk extender tolerated substantial concentrations of Tempo and Tempol compared with toxic effects in WM (P0.05). Therefore, optimal combinations of antioxidants tested here may have more useful applications in organizations using an egg yolk-based semen extender. |
| Databáze: | OpenAIRE |
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