Local immune response in the microenvironment of CIN2–3 with and without spontaneous regression
| Autor: | Ivar Skaland, Anais Malpica, Arnold-Jan Kruse, Emiel A. M. Janssen, Jan P. A. Baak, Irene T Øvestad, Einar Gudlaugsson |
|---|---|
| Přispěvatelé: | Obstetrie & Gynaecologie, RS: GROW - School for Oncology and Reproduction |
| Rok vydání: | 2010 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Stromal cell Genotype HPV genotype Uterine Cervical Neoplasms cervical intraepithelial neoplasia Biology Cervical intraepithelial neoplasia Polymerase Chain Reaction immune response Pathology and Forensic Medicine Young Adult Lymphocytes Tumor-Infiltrating Immune system Stroma Image Processing Computer-Assisted medicine Humans IL-2 receptor Papillomaviridae Retrospective Studies Papillomavirus Infections FOXP3 CIN regression Middle Aged Prognosis Uterine Cervical Dysplasia medicine.disease Immunohistochemistry female genital diseases and pregnancy complications Neoplasm Regression Spontaneous Female CD8 |
| Zdroj: | Modern Pathology, 23(9), 1231-1240. Nature Publishing Group |
| ISSN: | 0893-3952 |
| DOI: | 10.1038/modpathol.2010.109 |
| Popis: | Fifteen to thirty percent of cases with histologically confirmed CIN2-3 in cervical biopsies regress spontaneously (ie, show CIN1 or less in the follow-up cervical cone). The balance between immune-reactive cells from the host and high-risk human papillomavirus (hrHPV) genotypes may provide a biological explanation for this phenomenon. We retrospectively studied 55 cases of CIN2-3 in a cervical biopsy with subsequent cervical cone to assess whether hrHPV genotypes (by AMPLICOR and Linear Array tests) CD4, CD8, CD25, CD138 and Foxp3 cells (by quantitative immunohistochemistry) in the cervical biopsies can predict regression (defined as CIN1 or less in the follow-up cone biopsy). Eighteen percent of the CIN2-3 cases regressed (median biopsy-cervical cone time interval: 12.0 weeks, range: 5.0-34.1 weeks). HPV-16 correlated with low CD8(+) and high CD25(+). None of the regressing CIN2-3 lesions contained HPV-16. The regressing CIN2-3 lesions had lower numbers of stromal CD138(+) and higher numbers of stromal CD8(+) cells; higher stromal and intra-epithelial ratios of CD4(+)/CD25(+) cells; higher ratios of CD8(+)/CD25(+) cells and lower ratios of CD8(+)/CD4(+), CD138(+)/Foxp3(+) and CD25(+)/Foxp3(+) cells in the stroma. With multivariate survival analysis, stromal CD8(+) cell numbers, CD4(+)/CD25(+) cell ratios and CD138(+) cell numbers are found to be independent regression predictors. In conclusion, in non-HPV-16 CIN2-3 lesions, assessing stromal immune cells can be a useful prognostic indicator of regression or persistence. Modern Pathology (2010) 23, 1231-1240; doi:10.1038/modpathol.2010.109; published online 28 May 2010 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|