Tumors Associated With Oncogenic Osteomalacia Express Genes Important in Bone and Mineral Metabolism
| Autor: | Stephen L. Madden, Brian P. Cook, Justin Cho, Susan C. Schiavi, Joseph M. Petroziello, John Vassiliadis, Suzanne M. Jan de Beur, Partha Manavalan, Scott Estes, Michael A. Levine, Dana Barberio, Rajiv Kumar, Richard Finnegan |
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| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Calcitriol Bone disease Endocrinology Diabetes and Metabolism Mesenchyme Bone Neoplasms Biology Bone and Bones Gene expression medicine Humans Orthopedics and Sports Medicine Serial analysis of gene expression DNA Primers Osteomalacia Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Reproducibility of Results Phosphorus medicine.disease Oncogenic osteomalacia medicine.anatomical_structure Parathyroid Hormone Cancer research Calcium Hypophosphatemia medicine.drug |
| Zdroj: | Journal of Bone and Mineral Research. 17:1102-1110 |
| ISSN: | 0884-0431 |
| Popis: | Oncogenic osteomalacia (OOM) is associated with primitive mesenchymal tumors that secrete phosphaturic factors resulting in low serum concentrations of phosphate and calcitriol, phosphaturia, and defective bone mineralization. To identify overexpressed genes in these tumors, we compared gene expression profiles of tumors resected from patients with OOM and histologically similar control tumors using serial analysis of gene expression (SAGE). Three hundred and sixty-four genes were expressed at least twofold greater in OOM tumors compared with control tumors. A subset of 67 highly expressed genes underwent validation with an extended set of OOM and control tumors using array analysis or reverse-transcription polymerase chain reaction (RT-PCR). Ten of these validated genes were consistently overexpressed in all OOM tumors relative to control tumors. Strikingly, genes with roles in bone matrix formation, mineral ion transport, and bone mineralization were highly expressed in the OOM tumors. |
| Databáze: | OpenAIRE |
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