GABA tea attenuates cardiac apoptosis in spontaneously hypertensive rats (SHR) by enhancing PI3K/Akt-mediated survival pathway and suppressing Bax/Bak dependent apoptotic pathway
| Autor: | Vijaya Padma Viswanadha, Kuan Ho Lin, Bih Cheng Chen, Ray Jade Chen, Li Jen Yeh, Ruey Lin Chang, Hsueh Fang Wang, Meng Yu Hung, Sudhir Pandey, Chih Yang Huang |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Heart Ventricles Health Toxicology and Mutagenesis Apoptosis 030204 cardiovascular system & hematology Management Monitoring Policy and Law Pharmacology Toxicology Phosphatidylinositol 3-Kinases 03 medical and health sciences 0302 clinical medicine Rats Inbred SHR medicine Animals Insulin-Like Growth Factor I Protein kinase B gamma-Aminobutyric Acid PI3K/AKT/mTOR pathway bcl-2-Associated X Protein TUNEL assay Tea Caspase 3 Chemistry Receptors Somatomedin General Medicine medicine.disease Green tea Rats Blot bcl-2 Homologous Antagonist-Killer Protein 030104 developmental biology nervous system Heart failure Hypertension Proto-Oncogene Proteins c-akt Cardiomyocyte apoptosis Signal Transduction |
| Zdroj: | Environmental Toxicology. 33:789-797 |
| ISSN: | 1520-4081 |
| Popis: | Cardiomyocyte apoptosis is the major risk factor for the development of heart failure (HF). The purpose of this study was to evaluate the effects of Gamma-aminobutyric acid (GABA) tea on hypertension-induced cardiac apoptotic pathways in spontaneously hypertensive rats (SHR). In order to reveal the mechanisms, 36 male SHR at eight weeks of age, 200 g were divided into six groups. One group was fed water as a control group. Other rats were administered one of the following treatments: GABA tea at dose 150 and 300 mg/kg/day as low GABA tea (LGT) and high GABA tea (HGT) groups, respectively, pure GABA at dose 150 and 300 mg/kg/day as LG and HG groups, respectively, green tea (GT) as control of LGT and HGT groups. After 12 weeks, cardiac tissues were analyzed by histological analysis, western blotting, and TUNEL assays. GABA tea, GT, and pure GABA decreased hypertension-induced cardiac abnormalities, including abnormal myocardial architecture. In addition, GABA tea, GT, and pure GABA dramatically increased anti-apoptotic protein, Bcl2. Furthermore, GABA tea, GT, and pure GABA also decreased activated-caspase 9 and activated-caspase 3. Additionally, the survival associated protein IGF-I and PI3K/Akt were enhanced in cardiac tissues upon treatment. Our results showed an optimistic anti-apoptotic and pro-survival effects of GABA tea treatment against hypertensive rat hearts. |
| Databáze: | OpenAIRE |
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