Nucleotide sequence of the thymidine kinase gene of raccoon poxvirus
| Autor: | Cyril Lutze-Wallace, Manmohan Sidhu, Andreas Kappeler |
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| Rok vydání: | 1995 |
| Předmět: |
Genes
Viral viruses Molecular Sequence Data Vaccinia virus Biology Thymidine Kinase Homology (biology) Virus chemistry.chemical_compound Virology Sequence Homology Nucleic Acid Chlorocebus aethiops Genetics Animals Amino Acid Sequence Molecular Biology Vero Cells Base Sequence Sequence Homology Amino Acid Poxviridae Nucleic acid sequence General Medicine Molecular biology Open reading frame chemistry Thymidine kinase DNA Viral Nucleic acid Vero cell Raccoons Vaccinia |
| Zdroj: | Virus genes. 10(1) |
| ISSN: | 0920-8569 |
| Popis: | Using primers directed against the thymidine kinase gene of vaccinia virus, PCR products were generated from nucleic acids extracted from raccoon poxvirus-infected Vero cells. The PCR products were consistent in size with the expected products from vaccinia virus. Nucleotide sequence determination revealed that the raccoon pox thymidine kinase gene and flanking regions were 84.3% homologous to the corresponding sequences of vaccinia virus. At the amino acid level, an open reading frame coding for a polypeptide of 177 amino acids was found with 87% homology to thymidine kinase of vaccinia. |
| Databáze: | OpenAIRE |
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