Sensitivity of Yeast Mutants Deficient in Mitochondrial or Vacuolar ABC Transporters to Pathogenesis-Related Protein TcPR-10 of Theobroma cacao
Autor: | Cristina Pungartnik, Martin Brendel, Thayná Barreto, Louise R. Barreto, Sônia Cristina Oliveira Melo |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Programmed cell death pathogenesis related protein Mutant Saccharomyces cerevisiae ATP-binding cassette transporter Vacuole 01 natural sciences General Biochemistry Genetics and Molecular Biology Moniliophthora perniciosa 03 medical and health sciences oxidative stress PR-10 lcsh:QH301-705.5 Pathogenesis-related protein General Immunology and Microbiology biology biology.organism_classification Molecular biology Yeast 030104 developmental biology ABC transporters lcsh:Biology (General) General Agricultural and Biological Sciences 010606 plant biology & botany |
Zdroj: | Biology, Vol 7, Iss 2, p 35 (2018) Biology Volume 7 Issue 2 |
ISSN: | 2079-7737 |
Popis: | Pathogenesis-related proteins (PRs) are induced in plants after infection by pathogens and/or abiotic stress. Among these proteins, the family 10 (PR-10) influences the biosynthesis of secondary metabolites and shows antimicrobial ribonuclease activity. TcPR-10p (Pathogenesis-related Protein 10 of Theobroma cacao) was isolated from resistant and susceptible Moniliophthora perniciosa cacao cultivars. Cell survival with Saccharomyces cerevisiae mutant lines deficient in ATP-binding cassette (ABC) transporter proteins indicated the influence on resistance to TcPR-10p. Proteins of the ABC transport type are considered important in the process of resistance to antimicrobials and toxins. Thus, the objective of this work was to observe the sensitivity of ABC transporter yeast mutants in the presence of the TcPR-10p. Chronic exposure of S. cerevisiae mitochondrial (BYatm1&Delta and BYmdl1&Delta ) and vacuole (BYnft1&Delta BYvmr1&Delta BYybt1&Delta BYycf1&Delta and BYbpt1&Delta ) ABC transporter mutants to TcPR-10p (3 &mu g/mL, 0, 6, 12 and 24 h) was performed. Two TcPR-10p sensitive strains (BYmdl1&Delta and BYnft1&Delta ) were submitted to a fluorescence test with the fluorogenic dihydroethidium (DHE), to visualize the presence of oxidative stress in the cells. Oxidative stress-increased sensitivity was confirmed by flow cytometry indicating induced cell death either via apoptosis or necrosis. This yeast data combined with previous data of literature (of M. perniciosa sensitivity to TcPR-10p) show that increased sensitivity to TcPR-10p in these mutants could be due to the TcPR10p-generated higher levels of intracellular reactive oxygen species (ROS), leading to increased cell death either via necrosis or apoptosis. |
Databáze: | OpenAIRE |
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