Induction of CAMEL/NY-ESO-ORF2-specific CD8+ T cells upon stimulation with dendritic cells infected with a modified Ad5 vector expressing a chimeric Ad5/35 fiber
| Autor: | Johanna M M van Oers, Jaap Goudsmit, Stefan Kostense, Caroline E. van der Minne, Menzo J. E. Havenga, Elisabeth H. Slager, Marieke Griffioen, Susanne Osanto |
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| Přispěvatelé: | Amsterdam institute for Infection and Immunity, General Internal Medicine |
| Rok vydání: | 2004 |
| Předmět: |
Proteasome Endopeptidase Complex
endocrine system Cancer Research Recombinant Fusion Proteins medicine.medical_treatment T cell Genetic Vectors CD8-Positive T-Lymphocytes Biology Epitope Adenoviridae HLA-B7 Antigen Interleukin 21 Antigen Antigens Neoplasm Multienzyme Complexes Cell Line Tumor Chlorocebus aethiops medicine Animals Humans Cytotoxic T cell Molecular Biology HLA-A Antigens Interleukins Membrane Proteins Dendritic Cells Genetic Therapy Immunotherapy Virology Molecular biology Tumor antigen Cysteine Endopeptidases medicine.anatomical_structure Antigens Surface COS Cells Molecular Medicine Peptides Epitope Mapping CD8 |
| Zdroj: | Cancer gene therapy, 11(3), 227-236. Nature Publishing Group |
| ISSN: | 1476-5500 0929-1903 |
| DOI: | 10.1038/sj.cgt.7700674 |
| Popis: | Delivery of the full-length tumor antigen might be more successful in immunotherapy than single peptides and has the advantage that patients no longer need to be selected for their HLA type. In this study, we tested the in vitro induction of CAMEL/NY-ESO-ORF2-specific T cells by dendritic cells infected with an adenovirus (Ad) type 5 vector containing the fiber shaft and knob of human serotype Ad35 (Ad5F35 vector). Our data show induction of CD8(+) T cells specific for the known HLA-A(*)0201-binding CAMEL/NY-ESO-ORF2(1-11) epitope by DC infected with Ad5F35-CAMEL, but not by DC pulsed with the recombinant CAMEL protein. In one healthy donor, even CD8(+) T cells specific for a new HLA-B7-binding CAMEL/NY-ESO-ORF2(46-54) epitope were raised. In conclusion, the in vitro induction of CAMEL/NY-ESO-ORF2-specific CD8(+) T cells in healthy donors by DC infected with Ad5F35-CAMEL strongly supports further investigation of the Ad5F35 vector as a vehicle for gene transfer into DC for the generation of tumor antigen-specific CD8(+) T cell responses in vivo. |
| Databáze: | OpenAIRE |
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