Advantages of automation in plasma sample preparation prior to HPLC/MS/MS quantification: Application to the determination of cilazapril and cilazaprilat in a bioequivalence study
| Autor: | Yannis L. Loukas, Yannis Dotsikas, Georgios-Stefanos Soumelas, Filomila Kolocouri, Constantinos Apostolou, Constantinos Kousoulos |
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| Předmět: |
Calibration curve
Electrospray ionization Angiotensin-Converting Enzyme Inhibitors Cilazapril Sensitivity and Specificity High-performance liquid chromatography Specimen Handling Analytical Chemistry Cilazaprilat Automation Drug Stability Tandem Mass Spectrometry medicine Humans Environmental Chemistry Sample preparation Chromatography High Pressure Liquid Active metabolite Pharmacology Chromatography Molecular Structure Chemistry Selected reaction monitoring Therapeutic Equivalency Agronomy and Crop Science Food Science medicine.drug |
| Zdroj: | Scopus-Elsevier |
| Popis: | An HPLC/MS/MS method characterized by complete automation and high throughput was developed for the determination of cilazapril and its active metabolite cilazaprilat in human plasma. All sample preparation and analysis steps were performed by using 2.2 mL 96 deep-well plates, while robotic liquid handling workstations were utilized for all liquid transfer steps, including liquid–liquid extraction. The whole procedure was very fast compared to a manual procedure with vials and no automation. The method also had a very short chromatographic run time of 1.5 min. Sample analysis was performed by RP-HPLC/MS/MS with positive electrospray ionization using multiple reaction monitoring. The calibration curve was linear in the range of 0.500–300 and 0.250–150 ng/mL for cilazapril and cilazaprilat, respectively. The proposed method was fully validated and proved to be selective, accurate, precise, reproducible, and suitable for the determination of cilazapril and cilazaprilat in human plasma. Therefore, it was applied to a bioequivalence study after per os administration of 2.5 mg tablet formulations of cilazapril. |
| Databáze: | OpenAIRE |
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