| Autor: |
Ruimin, Tang, Chunsheng, Shao, Liangjian, Chen, Li, Yi, Bo, Zhang, Jiangjie, Tang, Weina, Ma |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biomaterials Advances. 137:212864 |
| ISSN: |
2772-9508 |
| DOI: |
10.1016/j.bioadv.2022.212864 |
| Popis: |
Osseointegration between implants and bone tissue lays the foundation for the long-term stability of implants. The incorporation of a porous structure and local slow release of siRNA to silence casein kinase-2 interacting protein-1 (CKIP-1), a downregulator of bone formation, is expected to promote osseointegration. Here, porous implants with a porous outer layer and dense inner core were prepared by metal coinjection molding (MIM). Mg-doped calcium phosphate nanoparticles (CaPNPs)-grafted arginine-glycine-aspartate cell adhesion sequence (RGD) and transcribed activator (TAT) (MCPRT)/CKIP-1 siRNA complex and polylysine (PLL) were coated onto the surface of the porous implants by layer-by-layer (LBL) self-deposition. The in vitro results showed that the MCPRT-siRNA coating promoted MG63 cell adhesion and proliferation, enhanced the protein expressions (ALP and OC) and bone formation-related gene expression (OPN, OC and COL-1α) in vitro. The in vivo results demonstrated that the porous structure enhanced bone ingrowth and that the local slow release of MCPRT-siRNA accelerated new bone formation at the early stage. The porous structure coupled with local CKIP-1 siRNA delivery constitutes a promising approach to achieve faster and stronger osseointegration for dental implants. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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