Rapid Detection of Tumor Necrosis Factor-Alpha Using Quantum Dot-Based Optical Aptasensor
| Autor: | Shreya Chaudhry, Mitra Dutta, Michael A. Stroscio, Shreya Ghosh, Debopam Datta |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Photoluminescence Aptamer Biomedical Engineering Analytical chemistry Pharmaceutical Science Medicine (miscellaneous) Alpha (ethology) Bioengineering Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences Quantum Dots Fluorescence Resonance Energy Transfer Humans Electrical and Electronic Engineering Fluorescent Dyes Detection limit chemistry.chemical_classification Quenching (fluorescence) Tumor Necrosis Factor-alpha Aptamers Nucleotide 021001 nanoscience & nanotechnology 0104 chemical sciences Computer Science Applications chemistry Transferrin Quantum dot 0210 nano-technology Selectivity Biotechnology |
| Zdroj: | IEEE Transactions on NanoBioscience. 17:417-423 |
| ISSN: | 1558-2639 1536-1241 |
| Popis: | This paper reports an optical “TURN OFF” aptasensor, which is comprised of a deoxyribonucleic acid aptamer attached to a quantum dot on the $5^{\prime }$ terminus and gold nanoparticle on the $3^{\prime }$ terminus. The photoluminescence intensity is observed to decrease upon progressive addition of the target protein tumor necrosis factor-alpha (TNF- $\alpha $ ) to the sensor. For PBS-based TNF- $\alpha $ samples, the beacon exhibited 19%–20% quenching at around 22 nM concentration. The photoluminescence intensity and the quenching efficiency showed a linear decrease and a linear increase, respectively, between 0 to 22.3 nM TNF- $\alpha $ . The detection limit of the sensor was found to be 97.2 pM. Specificity test results determined that the sensor has higher selectivity toward TNF- $\alpha $ than other control proteins such as C—reactive protein, albumin, and transferrin. The beacon successfully detected different concentrations of TNF- $\alpha $ in human serum-based samples exhibiting around 10% quenching efficiency at 12.5 nM of the protein. |
| Databáze: | OpenAIRE |
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