Pluripotent stem cells as a source of osteoblasts for bone tissue regeneration
| Autor: | Joy Y. Wu, Hui Zhu, Srilatha Swami, Takaharu Kimura |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetically modified mouse musculoskeletal diseases Pluripotent Stem Cells Bone Regeneration Cell Green Fluorescent Proteins Induced Pluripotent Stem Cells Biophysics Bioengineering Biology Bone tissue Regenerative medicine Article Cell Line Biomaterials 03 medical and health sciences Mice Tissue engineering medicine Animals Induced pluripotent stem cell Osteoblasts Regeneration (biology) Skull Cell Differentiation Mouse Embryonic Stem Cells Embryonic stem cell Cell biology Up-Regulation 030104 developmental biology medicine.anatomical_structure Mechanics of Materials Ceramics and Composites Transcriptome |
| Zdroj: | Biomaterials. 196 |
| ISSN: | 1878-5905 |
| Popis: | Appropriate and abundant sources of bone-forming osteoblasts are essential for bone tissue engineering. Pluripotent stem cells can self-renew and thereby offer a potentially unlimited supply of osteoblasts, a significant advantage over other cell sources. We generated mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from transgenic mice expressing rat 2.3 kb type I collagen promoter-driven green fluorescent protein (Col2.3GFP), a reporter of the osteoblast lineage. We demonstrated that Col2.3GFP ESCs and iPSCs can be successfully differentiated to osteoblast lineage cells that express Col2.3GFP in vitro. We harvested GFP+ osteoblasts differentiated from ESCs. Genome wide gene expression profiles validated that ESC- and iPSC-derived osteoblasts resemble calvarial osteoblasts, and that Col2.3GFP expression serves as a marker for mature osteoblasts. Our results confirm the cell identity of ESC- and iPSC-derived osteoblasts and highlight the potential of pluripotent stem cells as a source of osteoblasts for regenerative medicine. |
| Databáze: | OpenAIRE |
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