Serodiagnosis of sparganosis by ELISA using recombinant cysteine protease of Spirometra erinaceieuropaei spargana
| Autor: | Xi Zhang, Jing Cui, Li Na Liu, Peng Jiang, Ruo Dan Liu, Rui Zhe He, Zhong Quan Wang, Jian Zhou |
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| Rok vydání: | 2014 |
| Předmět: |
Sparganosis
Helminthiasis Antibodies Helminth Spirometra erinaceieuropaei Enzyme-Linked Immunosorbent Assay medicine.disease_cause Sensitivity and Specificity law.invention Sparganum Mice Antigen law Cysteine Proteases medicine Escherichia coli Animals Serologic Tests Spirometra Mice Inbred BALB C General Veterinary biology General Medicine medicine.disease biology.organism_classification Cysteine protease Molecular biology Recombinant Proteins Infectious Diseases Insect Science Antigens Helminth Immunoglobulin G biology.protein Recombinant DNA Parasitology Female Antibody |
| Zdroj: | Parasitology research. 114(2) |
| ISSN: | 1432-1955 |
| Popis: | The Spirometra erinaceieuropaei cysteine protease (SeCP) gene encoding a 36 kDa protein was expressed in Escherichia coli, and the potential of recombinant SeCP protein (rSeCP) as an antigen for the serodiagnosis of sparganosis was investigated by ELISA and compared with those of ELISA with sparganum excretory–secretory (ES) antigens. The sensitivity of rSeCP-ELISA and ES-ELISA was 96.67 % (29/30) and 100 % (30/30) respectively, for the detection of anti-sparganum IgG antibodies in sera of the experimentally infected mice (P > 0.05), and the specificities of both ELISA were 100 % (77/77). In heavily, moderately, and lightly infected mice (five, three, and one larvae per mouse), anti-sparganum antibodies were firstly detected by rSeCP-ELISA at 10–12 days post-infection (dpi), respectively, and then continued to increase with a detection rate of 100 % at 14–22 dpi. In three groups of infected mice, the anti-sparganum antibody levels at different times after infection were statistically different (P |
| Databáze: | OpenAIRE |
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