Synchrotron-based FTIR spectra of stained single cells. Towards a clinical application in pathology
| Autor: | Christophe Sandt, Josep Sulé-Suso, Gill Douce, Ka-Po Lam, Valérie Untereiner, Paul Dumas, Gary Parkes, Florence Draux, Ganesh D. Sockalingum, Ying Yang, Michel Manfait, Dave Collins, Jacek K. Pijanka, Daniel Gey van Pittius, Achim Kohler |
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| Přispěvatelé: | Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Lung Neoplasms [SDV]Life Sciences [q-bio] 02 engineering and technology Haematoxylin 01 natural sciences Cell Line Pathology and Forensic Medicine Ftir spectra chemistry.chemical_compound Cell Line Tumor Spectroscopy Fourier Transform Infrared medicine Humans Malignant cells Hematoxylin Lung cancer Lung Molecular Biology ComputingMilieux_MISCELLANEOUS Cell studies Pathology Clinical Staining and Labeling Eosin Chemistry 010401 analytical chemistry Reproducibility of Results Cancer Cell Biology 021001 nanoscience & nanotechnology medicine.disease Amides Lipids 3. Good health 0104 chemical sciences Staining Eosine Yellowish-(YS) 0210 nano-technology Synchrotrons |
| Zdroj: | Laboratory Investigation Laboratory Investigation, Nature Publishing Group, 2010, 90 (5), pp.797-807. ⟨10.1038/labinvest.2010.8⟩ |
| ISSN: | 0023-6837 1530-0307 |
| DOI: | 10.1038/labinvest.2010.8 |
| Popis: | Over the last few years, FTIR spectroscopy has become a potential analytical method in tissue and cell studies for cancer diagnosis. This has opened a way towards clinical applications such as a tool that would scan samples to assess the presence or absence of malignant cells in biopsies, or as an aid to help pathologists to better characterise those cells that are suspicious but not diagnostic for cancer. The latter application has the problem that in order to assess these cells pathologists would have already dealt with stained samples. Therefore, it is important to understand how staining would affect the spectra of cells. To this purpose, we have conducted this study in order to clarify, first, how haematoxylin and eosin (H&E) and Papanicolau (Pap) stainings affect the spectra of single cells and, second, whether FTIR spectroscopy could differentiate between stained lung cancer cells and their normal counterparts. Furthermore, different cell preparations (cytospin, and smear) used in cytological diagnosis were assessed. Experiments performed using a bright infrared (IR) source (synchrotron) showed that both H&E and Pap staining induced marked changes in the lipid and amide-II band regions. Despite this, FTIR spectroscopy of already stained cells is capable of differentiating between lung cancer cells and their normal counterparts. The clinical applications of this methodology are discussed. |
| Databáze: | OpenAIRE |
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