Enzymic activities and gene expression of enzymes of the acyl-CoA elongase during rapeseed development
| Autor: | René Lessire, Claude Cassagne, Juliette Puyaubert, W. Dieryck, Thomas Roscoe, Bertrand Garbay, Patricia Costaglioli |
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| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Rapeseed ATP synthase biology Reverse Transcriptase Polymerase Chain Reaction food and beverages Gene Expression Regulation Developmental Brassica Biochemistry Enzyme assay Gene Expression Regulation Enzymologic chemistry.chemical_compound Enzyme Mrna level chemistry Erucic acid Gene Expression Regulation Plant Gene expression Seeds biology.protein Acyl-Carrier Protein S-Malonyltransferase Acyl-CoA elongase Acyltransferases |
| Zdroj: | Biochemical Society transactions. 28(6) |
| ISSN: | 0300-5127 |
| Popis: | Enzymic activities and gene expression of oleoylCoA elongase were studied during seed development using two different rapeseed cultivars, high-erucic-acid rapeseed (HEAR) and low-erucic-acid rapeseed (LEAR). The overall elongase activities were maximal in HEAR between the fourth and eighth weeks after pollination (WAP) and absent in LEAR. The 3-ketoacyl-CoA synthase (condensing enzyme, CE) mRNA levels and the developmental profiles in the two cultivars were different since maximal expression levels were detected in HEAR and LEAR at WAP 4 and WAP 6, respectively. Anti-CE antibodies revealed two proteins of 60 and 67 kDa in both cultivars and an additional reacting protein of 57 kDa in HEAR. |
| Databáze: | OpenAIRE |
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