Chronic maternal interleukin-17 and autism-related cortical gene expression, neurobiology, and behavior
| Autor: | Hanna E. Stevens, Benjamin Hing, Jessica Jolynn Dewitt, Sabrina M Scroggins, Akanksha Sri Satya Chilukuri, Serena B. Gumusoglu |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Autism Spectrum Disorder Offspring Neuroimmunology Gene Expression Biology Article Pathogenesis 03 medical and health sciences 0302 clinical medicine Pregnancy Internal medicine Gene expression medicine Animals Autistic Disorder Pharmacology Behavior Animal Interleukin-17 Correction Autism spectrum disorders medicine.disease Phenotype 030227 psychiatry Psychiatry and Mental health Endocrinology MRNA Sequencing Autism spectrum disorder Prenatal Exposure Delayed Effects Autism Female Experimental organisms 030217 neurology & neurosurgery |
| Zdroj: | Neuropsychopharmacology |
| ISSN: | 1740-634X 0893-133X |
| Popis: | Chronic inflammation during pregnancy (e.g., preeclampsia, diabetes) is linked to increased risk for offspring neurodevelopmental disorders such as autism spectrum disorder (ASD). However, mediators of such exposures that could be targeted with maternal intervention are unclear, as few chronic gestational inflammation models have been tested. One potential mediator is interleukin-17 (IL-17), a pro-inflammatory cytokine implicated in neurodevelopmental disorders and gestational disease. To test chronic maternal IL-17 impacts on offspring, C57BL/6J dams were administered IL-17A continuously throughout pregnancy. Offspring were assessed for body weight; cortical volume, gene expression, and cellular composition; and adult behavior. IL-17A-condition offspring exhibited decreased somatic and cortical size at embryonic day 18 (E18) and as adults. mRNA sequencing of E18 cortex revealed 320 differentially expressed genes in males, but none in females. These were significantly enriched for ASD (Simons Foundation Autism Research Initiative), synaptic, and cell cycle genes. By adulthood, neocortical glial cell density and gene expression were decreased, while GABAergic synaptic gene expression was increased in males. Furthermore, IL-17A-condition male but not female offspring exhibited reduced anxiety-like behavior. Social approach deficits in males were negatively correlated with neocortical GABAergic synaptic gene expression. Chronic gestational IL-17A was sufficient to cause ASD-like phenotypes early and persistently in male offspring. This echoes the male bias, altered cortical development, and behavioral findings in ASD, suggesting that chronic maternal IL-17 contributes to offspring ASD pathogenesis. Furthermore, the trajectory from embryonically dysregulated synaptic and cell cycle genes to disrupted adult glia, inhibitory synapses, and behavior suggests a mechanism for chronic maternal IL-17 effects on offspring. |
| Databáze: | OpenAIRE |
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