Detection of carcinoembryonic antigens using a wavy gold–silver alloy nanoplate enhanced surface plasmon resonance imaging biosensor

Autor:	Jiangling Wu, Qiongyuan Zhang, Lina Kang, Xiaotian Wu, Daikun Li, Yi Wang, Yu Huang, Jianjiang Xue
Rok vydání:	2022
Předmět:	Silver General Chemical Engineering General Engineering Gold Alloys Metal Nanoparticles Gold Biosensing Techniques Surface Plasmon Resonance Carcinoembryonic Antigen Analytical Chemistry
Zdroj:	Analytical Methods. 14:4713-4720
ISSN:	1759-9679 1759-9660
DOI:	10.1039/d2ay01523d
Popis:	Carcinoembryonic antigen (CEA) is regarded as a promising broad spectrum tumor biomarker for clinical diagnosis, progression, and prognosis. Surface plasmon resonance imaging (SPRi) was considered as one of the powerful tools for immunoassay with advantages of label-free, real-time detection with high-throughput. Herein, wavy gold-silver alloy nanoplates functionalized with anti-CEA antibodies providing high protein loading capacity and high mass are used as signal enhancers for CEA detection through SPRi sandwich assay. The present method exhibits a dynamic range for CEA determination from 0.1 to 312.5 ng mL
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a1a2362f02161c56477773f27ddb6d9 https://doi.org/10.1039/d2ay01523d Zobrazit plný text záznamu