Morphological and immunohistochemical identification of epithelial-to-mesenchymal transition in clinical prostate cancer
| Autor: | Esther I. Verhoef, Kimberley Kolijn, Geert J.L.H. van Leenders |
|---|---|
| Přispěvatelé: | Pathology |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Vimentin Metastasis Prostate cancer Downregulation and upregulation SDG 3 - Good Health and Well-being Cell Line Tumor Biomarkers Tumor medicine Humans Epithelial–mesenchymal transition N-cadherin Aged biology Cadherin business.industry Mesenchymal stem cell Prostatic Neoplasms Middle Aged Cadherins prostate cancer medicine.disease Fibronectins Gene Expression Regulation Neoplastic Oncology ill-defined biology.protein Immunohistochemistry epithelial-to-mesenchymal transition Clinical Research Paper business Signal Transduction |
| Zdroj: | Oncotarget, 6(27), 24488-24498. Impact Journals LLC Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.4177 |
| Popis: | // Kimberley Kolijn 1 , Esther I. Verhoef 1 and Geert J.L.H. van Leenders 1 1 Department of Pathology, Erasmus Medical Centre, Rotterdam, The Netherlands Correspondence to: Kimberley Kolijn, email: // Keywords : epithelial-to-mesenchymal transition, ill-defined, N-cadherin, prostate cancer Received : March 06, 2015 Accepted : May 02, 2015 Published : May 19, 2015 Abstract Epithelial-to-mesenchymal transition (EMT) is a process known to be associated with aggressive tumor behavior, metastasis and treatment resistance. It is characterized by coincidental upregulation of mesenchymal markers such as vimentin, fibronectin and N-cadherin concurrent with E-cadherin downregulation. Studies on EMT are generally performed in cell lines and mouse models, while the histopathological and phenotypical properties in clinical prostate cancer (PCa) are still unclear. The objective of this study was to identify EMT in PCa patients. We demonstrated that N-cadherin, vimentin and fibronectin were generally not co-expressed in corresponding tumor regions. Immunofluorescent double stainings confirmed that co-expression of mesenchymal markers was uncommon, as we found no prostate cancer cells that co-expressed N-cadherin with fibronectin and only rare ( |
| Databáze: | OpenAIRE |
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