Genetic divergence between two sympatric species of the Lutzomyia longipalpis complex in the paralytic gene, a locus associated with insecticide resistance and lovesong production
| Autor: | Alexandre A. Peixoto, Nataly A. Souza, R. M. M. A. Lins |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Genetic Markers Species complex sandflies lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Molecular Sequence Data lcsh:QR1-502 Population genetics Locus (genetics) Genes Insect Polymerase Chain Reaction lcsh:Microbiology Sodium Channels Insecticide Resistance chemistry.chemical_compound Species Specificity Lutzomyia longipalpis Molecular marker Animals Leishmaniasis Genetics biology sympatric species Courtship insecticide resistance biology.organism_classification Sandfly Insect Vectors para Genetic divergence Animal Communication chemistry Amino Acid Substitution Genetic marker Sympatric speciation Drosophila Psychodidae |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 103, Issue: 7, Pages: 736-740, Published: NOV 2008 Memórias do Instituto Oswaldo Cruz., Vol 103, Iss 7, Pp 736-740 (2008) |
| Popis: | The sandfly Lutzomyia longipalpis s.l. is the main vector of American Visceral Leishmaniasis. L. longipalpis s.l. is a species complex but until recently the existence of cryptic sibling species among Brazilian populations was a controversial issue. A fragment of paralytic (para), a voltage dependent sodium channel gene associated with insecticide resistance and courtship song production in Drosophila, was isolated and used as a molecular marker to study the divergence between two sympatric siblings of the L. longipalpis complex from Sobral, Brazil. The results revealed para as the first single locus DNA marker presenting fixed differences between the two species in this locality. In addition, two low frequency amino-acid changes in an otherwise very conserved region of the channel were observed, raising the possibility that it might be associated with incipient resistance in this vector. To the best of our knowledge, the present study represents the first population genetics analysis of insecticide resistance genes in this important leishmaniasis vector. |
| Databáze: | OpenAIRE |
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